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Literature summary for 3.4.24.55 extracted from

  • Anastasi, A.; Knight, C.G.; Barrett, A.J.
    Characterization of the bacterial metalloendopeptidase pitrilysin by use of a continuous fluorescence assay (1993), Biochem. J., 290, 601-607.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.55

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Escherichia coli
2,6-pyridinedicarboxylic acid
-
 Escherichia coli
bacitracin especially in the presence of zinc Escherichia coli
Ca2+ 1 mM, weak, reactivates after inhibition with chelating agents Escherichia coli
Co2+ 1 mM Escherichia coli
DTT
-
 Escherichia coli
dynorphin A(1-13)
-
 Escherichia coli
EDTA
-
 Escherichia coli
Mn2+ 1 mM, weak, reactivates after inhibition with chelating agents Escherichia coli
additional information alpha-macroglobulin Escherichia coli
Tetraethylenepentamine
-
 Escherichia coli
Zincov
-
 Escherichia coli
Zn2+ 1 mM inhibits, but reactivates after inhibition with chelating agents Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0077
-
 (7-methoxycoumarin-4-yl)acetyl-Nle-Ala-Val-Lys-Tyr-Leu-Asn-Ser-Lys(2,4-dinitrophenyl)-Leu-Asp-D-Lys
-
 Escherichia coli
0.0127
-
 glucagon
-
 Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
 Escherichia coli
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ reactivation after inhibition with chelating agents Escherichia coli
Mn2+ reactivation after inhibition with chelating agents Escherichia coli
Zn2+ most effective metal ion in reactivation after inhibition by chelating agent Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
116000
-
 1 * 116000, SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 overexpressing strain
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(7-Methoxycoumarin-4-yl)acetyl-Nle-Ala-Val-Lys-Tyr-Leu-Asn-Ser-(2,4-dinitrophenyl)Lys-Leu-Asp-D-Lys + H2O
-
 Escherichia coli ?
-
 ?
(7-methoxycoumarin-4-yl)acetyl-Nle-Ala-Val-Lys-Tyr-Leu-Asn-Ser-Lys(2,4-dinitrophenyl)-Leu-Asp-D-Lys + H2O
-
 Escherichia coli ?
-
 ?
Glucagon + H2O
-
 Escherichia coli ?
-
 ?
Insulin B-chain + H2O small peptides down to 10 residues in length are cleaved more slowly, intact insulin is cleaved very slowly Escherichia coli ?
-
 ?
additional information the activity of the enzyme is confined to substrates smaller than proteins Escherichia coli ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 116000, SDS-PAGE Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.05
-
 glucagon
-
 Escherichia coli