Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Escherichia coli | |
2,6-pyridinedicarboxylic acid | - |
Escherichia coli | |
bacitracin | especially in the presence of zinc | Escherichia coli | |
Ca2+ | 1 mM, weak, reactivates after inhibition with chelating agents | Escherichia coli | |
Co2+ | 1 mM | Escherichia coli | |
DTT | - |
Escherichia coli | |
dynorphin A(1-13) | - |
Escherichia coli | |
EDTA | - |
Escherichia coli | |
Mn2+ | 1 mM, weak, reactivates after inhibition with chelating agents | Escherichia coli | |
additional information | alpha-macroglobulin | Escherichia coli | |
Tetraethylenepentamine | - |
Escherichia coli | |
Zincov | - |
Escherichia coli | |
Zn2+ | 1 mM inhibits, but reactivates after inhibition with chelating agents | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0077 | - |
(7-methoxycoumarin-4-yl)acetyl-Nle-Ala-Val-Lys-Tyr-Leu-Asn-Ser-Lys(2,4-dinitrophenyl)-Leu-Asp-D-Lys | - |
Escherichia coli | |
0.0127 | - |
glucagon | - |
Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Escherichia coli | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | reactivation after inhibition with chelating agents | Escherichia coli | |
Mn2+ | reactivation after inhibition with chelating agents | Escherichia coli | |
Zn2+ | most effective metal ion in reactivation after inhibition by chelating agent | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
116000 | - |
1 * 116000, SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
overexpressing strain | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(7-Methoxycoumarin-4-yl)acetyl-Nle-Ala-Val-Lys-Tyr-Leu-Asn-Ser-(2,4-dinitrophenyl)Lys-Leu-Asp-D-Lys + H2O | - |
Escherichia coli | ? | - |
? | |
(7-methoxycoumarin-4-yl)acetyl-Nle-Ala-Val-Lys-Tyr-Leu-Asn-Ser-Lys(2,4-dinitrophenyl)-Leu-Asp-D-Lys + H2O | - |
Escherichia coli | ? | - |
? | |
Glucagon + H2O | - |
Escherichia coli | ? | - |
? | |
Insulin B-chain + H2O | small peptides down to 10 residues in length are cleaved more slowly, intact insulin is cleaved very slowly | Escherichia coli | ? | - |
? | |
additional information | the activity of the enzyme is confined to substrates smaller than proteins | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 116000, SDS-PAGE | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
glucagon | - |
Escherichia coli |