KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0076 | - |
2-Aminobenzoyl-Thr-Glu-Lys-Leu-Val-2,4-dinitroanilinoethylamide | - |
Protobothrops flavoviridis | |
0.013 | - |
2-Aminobenzoyl-Phe-Ser-Pro-Met-Leu-2,4-dinitroanilinoethylamide | 2-aminobenzoyl-Gly-Phe-Arg-Leu-Leu-2,4-dinitroanilinoethylamide | Protobothrops flavoviridis | |
0.014 | - |
2-aminobenzoyl-Asn-Ala-Phe-Leu-Ala-2,4-dinitroanilinoethylamide | - |
Protobothrops flavoviridis | |
0.015 | - |
2-Aminobenzoyl-Ser-Pro-Met-Leu-2,4-dinitroanilinoethylamide | - |
Protobothrops flavoviridis | |
0.12 | - |
2-Aminobenzoyl-Ala-Gly-Leu-Ala-2,4-dinitroanilinoethylamide | - |
Protobothrops flavoviridis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Protobothrops flavoviridis | the major hemorrhagic component in the venom of Trimeresurus flavoviridis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Protobothrops flavoviridis | - |
Habu snake | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Protobothrops flavoviridis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-Aminobenzoyl-Ala-Gly-Leu-Ala-2,4-dinitroanilinoethylamide + H2O | peptide synthesized on the basis of sequences close to autoproteolysis cleavage sites, fluorogenic substrate | Protobothrops flavoviridis | 2-Aminobenzoyl-Ala-Gly + Leu-Ala-2,4-dinitroanilinoethylamide | - |
? | |
2-aminobenzoyl-Asn-Ala-Phe-Leu-Ala-2,4-dinitroanilinoethylamide + H2O | - |
Protobothrops flavoviridis | ? | - |
? | |
2-Aminobenzoyl-Asn-Ala-Pro-Leu-Ala-2,4-dinitroanilinoethylamide + H2O | peptide synthesized on the basis of sequences close to autoproteolysis cleavage sites, fluorogenic substrate | Protobothrops flavoviridis | 2-Aminobenzoyl-Asn-Ala-Pro + Leu-Ala-2,4-dinitroanilinoethylamide | - |
? | |
2-Aminobenzoyl-Gly-Phe-Arg-Leu-Leu-2,4-dinitroanilinoethylamide + H2O | peptide synthesized on the basis of sequences close to autoproteolysis cleavage sites, fluorogenic substrate | Protobothrops flavoviridis | 2-Aminobenzoyl-Gly-Phe-Arg + Leu-Leu-2,4-dinitroanilinoethylamide | - |
? | |
2-Aminobenzoyl-Phe-Ser-Pro-Met-Leu-2,4-dinitroanilinoethylamide + H2O | peptide synthesized on the basis of sequences close to autoproteolysis cleavage sites, fluorogenic substrate | Protobothrops flavoviridis | 2-Aminobenzoyl-Phe-Ser-Pro + Met-Leu-2,4-dinitroanilinoethylamide | - |
? | |
2-Aminobenzoyl-Ser-Pro-Met-Leu-2,4-dinitroanilinoethylamide + H2O | peptide synthesized on the basis of sequences close to autoproteolysis cleavage sites, fluorogenic substrate | Protobothrops flavoviridis | 2-Aminobenzoyl-Ser-Pro + Met-Leu-2,4-dinitroanilinoethylamide | - |
? | |
2-Aminobenzoyl-Thr-Glu-Lys-Leu-Val-2,4-dinitroanilinoethylamide + H2O | peptide synthesized on the basis of sequences close to autoproteolysis cleavage sites, fluorogenic substrate | Protobothrops flavoviridis | 2-Aminobenzoyl-Thr-Glu-Lys + Leu-Val-2,4-dinitroanilinoethylamide | - |
? | |
Metalloproteinase HR1A + H2O | MW 60000, bears autoproteolytic activity which is suppressed by more than 0.005 mM Ca2+ or 0.05 mM EDTA | Protobothrops flavoviridis | Hydrolyzed metalloproteinase HR1A | fragment MW 32000 from COOH-terminus and low MW fragments | ? | |
Metalloproteinase HR1B + H2O | MW 60000, bears autoproteolytic activity which is suppressed by more than 5 mM Ca2+ or EDTA | Protobothrops flavoviridis | Hydrolyzed metalloproteinase HR1B | fragment MW 34000 and low MW fragments | ? | |
additional information | 2-aminobenzoyl-Asn-Ala-Pro-Ser-Ala-2,4-dinitroanilinoethylamide, 2-aminobenzoyl-Ala-Thr-Asp-Ile-Val-2,4-dinitroanilinoethylamide, 2-aminobenzoyl-Gly-Phe-Arg-Leu-2,4-dinitroanilinoethylamide, 2-aminobenzoyl-Gly-Phe-Leu-Gly-Pro-2,4-dinitroanilinoethylamide | Protobothrops flavoviridis | ? | - |
? | |
additional information | the major hemorrhagic component in the venom of Trimeresurus flavoviridis | Protobothrops flavoviridis | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Protobothrops flavoviridis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
2-aminobenzoyl-Ser-Pro-Met-Leu-2,4-dinitroanilinoethylamide as substrate | Protobothrops flavoviridis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10.5 | half-maximal activity at pH 6 and 10.5, 2-aminobenzoyl-Ser-Pro-Met-Leu-2,4-dinitroanilinoethylamide as substrate | Protobothrops flavoviridis |