Crystallization (Comment) | Organism |
---|---|
Crotalus adamanteus | Crotalus adamanteus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 2 mM Zn2+ protects at 2 mM 2-mercaptoethanol, 2 mM Zn2+ partially protects at 10-50 mM 2-mercaptoethanol, 2 mM Ca2+ partially protects at 2-10 mM 2-mercaptoethanol, no protection at 50 mM 2-mercaptoethanol | Crotalus adamanteus | |
cysteine | 2 mM Zn2+ protects at 2 mM cysteine, protects partially at 10 mM cysteine, no protection at 20 mM cysteine | Crotalus adamanteus | |
EDTA | 2 mM Zn2+ protects at 0.5-1 mM EDTA, no protection at 2 mM EDTA, 2 mM Ca2+ partially protects at 0.5 mM EDTA, weakly at 1 mM EDTA, no protection at 2 mM EDTA; hide powder azure or alpha1-proteinase inhibitor as substrates | Crotalus adamanteus | |
GSH | 2 mM Zn2+ or Ca2+ protects at 2 mM GSH, partially protects at 10 mM GSH, no protection at 50 mM GSH | Crotalus adamanteus | |
additional information | no inhibition by diisopropyl fluorophosphate | Crotalus adamanteus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | metalloproteinase | Crotalus adamanteus | |
Zn2+ | zinc containing metalloproteinase | Crotalus adamanteus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
amino acid composition compared to proteinase I | Crotalus adamanteus |
23750 | - |
Crotalus adamanteus, calculated from amino acid content | Crotalus adamanteus |
24000 | - |
Crotalus adamanteus, PAGE | Crotalus adamanteus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Crotalus adamanteus | - |
Eastern diamondback rattlesnake | - |
Purification (Comment) | Organism |
---|---|
- |
Crotalus adamanteus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Crotalus adamanteus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Crotalus adamanteus |
Storage Stability | Organism |
---|---|
-18°C, in 0.05 M sodium borate, pH 8, 2 mM CaCl2, at least 6 months | Crotalus adamanteus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
casein + H2O | - |
Crotalus adamanteus | ? | - |
? | |
hide powder azure + H2O | - |
Crotalus adamanteus | ? | - |
? | |
human plasma alpha1-proteinase inhibitor + H2O | MW 54000, native and heat-denatured, the latter is more extensively hydrolyzed | Crotalus adamanteus | hydrolyzed and inactivated human plasma alpha1-proteinase inhibitor | MW 50000 and MW 4000 fragments | ? | |
additional information | no hydrolysis of benzoyl-L-Arg ethyl ester, benzoyl-L-Tyr ethyl ester | Crotalus adamanteus | ? | - |
? | |
Oxidized insulin B-chain + H2O | cleavage at Phe1-Val2, His5-Leu6, His10-Leu11, Ala14-Leu15, Leu15-Tyr16 and Tyr16-Leu17 | Crotalus adamanteus | ? | - |
? | |
Polyarginine + H2O | - |
Crotalus adamanteus | Hydrolyzed polyarginine | chain length 6-12 amino acids, not arginine monomers | ? | |
Polylysine + H2O | - |
Crotalus adamanteus | Hydrolyzed polylysine | chain length 6-12 amino acids, not lysine monomers | ? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 24000, Crotalus adamanteus, SDS-PAGE | Crotalus adamanteus |