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Literature summary for 3.4.24.42 extracted from

  • Fox, J.W.; Bjarnason, J.B.
    Atrolysins: metalloproteinases from Crotalus atrox venom (1995), Methods Enzymol., 248, 368-387.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Crotalus atrox

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Crotalus atrox

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Crotalus atrox
alpha2-Macroglobulin
-
Crotalus atrox
Amino acid hydroxamates
-
Crotalus atrox
Benzyloxycarbonyl-Leu-Gly-NHOH
-
Crotalus atrox
EDTA
-
Crotalus atrox

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ zinc metalloproteinase Crotalus atrox
Zn2+ 1 mol zinc/mol enzyme Crotalus atrox

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
they differ by a single amino acid substitution at position 181, Ala (Cc) or Asp (Cd) Crotalus atrox
24000
-
1 * 24000, Crotalus atrox Crotalus atrox

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Basement membranes surrounding capillaries + H2O Crotalus atrox together with atrolysins B and E member of the class P-I hemorrhagic toxins ?
-
?

Organism

Organism UniProt Comment Textmining
Crotalus atrox
-
Western diamondback rattlesnake
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information no glycoprotein Crotalus atrox

Purification (Commentary)

Purification (Comment) Organism
formerly Ht-c and Ht-d Crotalus atrox
from lyophilized crude venom Crotalus atrox
two isozymes Cc and Cd Crotalus atrox

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
Crotalus atrox
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-Aminobenzoyl-Ala-Gly-Leu-Ala 4-nitrobenzyl amide + H2O
-
Crotalus atrox ?
-
?
Basement membrane preparation + H2O
-
Crotalus atrox Soluble peptides
-
?
Basement membranes surrounding capillaries + H2O together with atrolysins B and E member of the class P-I hemorrhagic toxins Crotalus atrox ?
-
?
Collagen type IV + H2O basement membrane component, alpha1 (MW 185000) and alpha2 (MW 170000) chain Crotalus atrox Hydrolyzed collagen type IV
-
?
Fibrinogen + H2O
-
Crotalus atrox Hydrolyzed fibrinogen
-
?
Fibronectin + H2O
-
Crotalus atrox ?
-
?
Gelatin of collagen type I + H2O i.e. denatured collagen Crotalus atrox Hydrolyzed gelatin of collagen type I
-
?
Gelatin of collagen type III + H2O i.e. denatured collagen Crotalus atrox ?
-
?
Human alpha2-macroglobulin + H2O cleavage at Arg696-Leu697 Crotalus atrox ?
-
?
Laminin + H2O
-
Crotalus atrox ?
-
?
Leu-Val-Glu-Ala-Leu-Tyr-Leu + H2O cleavage at Ala-Leu Crotalus atrox Leu-Val-Glu-Ala + Leu-Tyr-Leu
-
?
additional information no hydrolysis of fibrin Crotalus atrox ?
-
?
additional information substrate digestion patterns differ from those of atrolysins A and E Crotalus atrox ?
-
?
Nidogen + H2O basement membrane component Crotalus atrox ?
-
?
Oxidized insulin B-chain + H2O
-
Crotalus atrox ?
-
?

Subunits

Subunits Comment Organism
monomer SDS-PAGE Crotalus atrox
monomer 1 * 24000, Crotalus atrox Crotalus atrox

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
pI: 6 (isozyme Cc), pI: 6.1 (isozyme Cd) Crotalus atrox