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Literature summary for 3.4.24.40 extracted from

  • Basu, B.; Apte, S.K.
    A novel serralysin metalloprotease from Deinococcus radiodurans (2008), Biochim. Biophys. Acta, 1784, 1256-1264.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline complete inhibition Deinococcus radiodurans
Ca2+ inhibitory above 0.1 mM Deinococcus radiodurans
Co2+ inhibitory above 5 mM Deinococcus radiodurans
EDTA complete inhibition Deinococcus radiodurans
EGTA complete inhibition Deinococcus radiodurans
additional information not inhibitory: PMSF, E-64 and pepstatin Deinococcus radiodurans
Zn2+ inhibitory above 0.025 mM Deinococcus radiodurans

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular and intracellular Deinococcus radiodurans
-
-
intracellular and extracellular Deinococcus radiodurans 5622
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ in presence of Ca2+, Zn2+ or Co2+ may recativate EDTA-inactivated protease. Ca2+ concentrations required are between 0.01 mM and 0.1 mM, inhibitory above 0.1 mM Deinococcus radiodurans
Co2+ in the presence of Ca2+, restoration of EDTA-inactivied protease. No reactivation in absence of Ca2+ Deinococcus radiodurans
Zn2+ in the presence of Ca2+, restoration of EDTA-inactivied protease. No reactivation in absence of Ca2+. Inhibitory above 0.025 mM Deinococcus radiodurans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
85000
-
x * 85000, SDS-PAGE Deinococcus radiodurans

Organism

Organism UniProt Comment Textmining
Deinococcus radiodurans
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gelatin + H2O
-
Deinococcus radiodurans ?
-
?
additional information no substrate: casein, bovine serum albumin, lysozyme, lactalbumin, lactoglobulin and thyroglobulin Deinococcus radiodurans ?
-
?

Subunits

Subunits Comment Organism
? x * 85000, SDS-PAGE Deinococcus radiodurans

Synonyms

Synonyms Comment Organism
DR2310 protease
-
Deinococcus radiodurans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Deinococcus radiodurans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50
-
Deinococcus radiodurans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Deinococcus radiodurans

pH Range

pH Minimum pH Maximum Comment Organism
4.5 9.5
-
Deinococcus radiodurans