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Literature summary for 3.4.24.40 extracted from

  • Decedue, C.J.; Broussard, E.A.; Larson, A.D.; Braymer, H.D.
    Purification and characterization of the extracellular proteinase of Serratia marcescens (1979), Biochim. Biophys. Acta, 569, 293-301.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline neither Ca2+, Zn2+ nor Fe2+ restores Serratia marcescens
EDTA Ca2+ (not) restores; Fe2+ (not); Zn2+ (not) Serratia marcescens
additional information
-
Serratia marcescens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Serratia marcescens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
amino acid composition Serratia marcescens
47500
-
1 * 47500, Serratia marcescens, SDS-PAGE Serratia marcescens
51900
-
Serratia marcescens, sedimentation equilibrium centrifugation Serratia marcescens

Organism

Organism UniProt Comment Textmining
Serratia marcescens
-
derepressed strain
-

Purification (Commentary)

Purification (Comment) Organism
to near homogeneity Serratia marcescens

Source Tissue

Source Tissue Comment Organism Textmining
culture supernatant
-
Serratia marcescens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Serratia marcescens

Storage Stability

Storage Stability Organism
-70°C, lyophilized Serratia marcescens enzyme, several months Serratia marcescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Aminoethylated lysozyme + H2O
-
Serratia marcescens ?
-
?
C-terminal octapeptide of glucagon + H2O cleavage sites Serratia marcescens ?
-
?
Carboxymethyl-beta-insulin + H2O cleavage sites Serratia marcescens ?
-
?
casein + H2O
-
Serratia marcescens ?
-
?
Glucagon + H2O cleavage sites Serratia marcescens ?
-
?
Hemoglobin + H2O
-
Serratia marcescens ?
-
?
additional information no substrates are small peptides Serratia marcescens ?
-
?
additional information minimum peptide size: hexapeptide Serratia marcescens ?
-
?
additional information no esterase activity: no substrates are N-benzoyl-L-Arg ethyl ester or N-benzoyl-L-Tyr ethyl esters Serratia marcescens ?
-
?
N-terminal hexapeptide of glucagon + H2O cleavage sites Serratia marcescens ?
-
?
Ribonuclease + H2O
-
Serratia marcescens ?
-
?
Tryptic insulin heptapeptide + H2O cleavage sites Serratia marcescens ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 47500, Serratia marcescens, SDS-PAGE Serratia marcescens

pH Range

pH Minimum pH Maximum Comment Organism
7.5 10.6
-
Serratia marcescens