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Literature summary for 3.4.24.38 extracted from

  • Matsuda, Y.; Yamasaki, A.; Saito, T.; Yamaguchi, T.
    Purification and characterization of cell wall lytic enzyme released by mating gametes of Chlamydomonas reinhardtii (1984), FEBS Lett., 166, 293-297.
No PubMed abstract available
Search for more literature for 3.4.24.38

General Stability

General Stability Organism
DTT stabilizes Chlamydomonas reinhardtii

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Chlamydomonas reinhardtii
alpha2-Macroglobulin
-
 Chlamydomonas reinhardtii
diethyldicarbonate
-
 Chlamydomonas reinhardtii
EDTA
-
 Chlamydomonas reinhardtii
EGTA
-
 Chlamydomonas reinhardtii
HgCl2
-
 Chlamydomonas reinhardtii
iodoacetate
-
 Chlamydomonas reinhardtii
additional information no inhibition by trypsin inhibitor, 6-aminohexanoic acid; pepstatin A Chlamydomonas reinhardtii
PCMB
-
 Chlamydomonas reinhardtii
PMSF
-
 Chlamydomonas reinhardtii

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activation, 0.75-1 mM Chlamydomonas reinhardtii
Mg2+ activation, 0.75-1 mM Chlamydomonas reinhardtii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
 x * 62000, Chlamydomonas reinhardtii, SDS-PAGE Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Cell wall protein + H2O Chlamydomonas reinhardtii cell wall lytic enzyme, reaction involved in cell fusion during mating of Chlamydomonas reinhardtii gametes ?
-
 ?

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii
-
 green alga, wild-type strain 137c
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Chlamydomonas reinhardtii

Purification (Commentary)

Purification (Comment) Organism
-
 Chlamydomonas reinhardtii

Source Tissue

Source Tissue Comment Organism Textmining
additional information mt+ (predominantly, ) and mt- gametes Chlamydomonas reinhardtii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Chlamydomonas reinhardtii

Storage Stability

Storage Stability Organism
-70°C, in Tris-acetate buffer, pH 7.5, 0.2 M NaCl, 2 mM DTT Chlamydomonas reinhardtii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Cell wall protein + H2O cell wall lytic enzyme, reaction involved in cell fusion during mating of Chlamydomonas reinhardtii gametes Chlamydomonas reinhardtii ?
-
 ?
Zoosporangia cell walls + H2O
-
 Chlamydomonas reinhardtii ?
-
 ?

Subunits

Subunits Comment Organism
? x * 62000, Chlamydomonas reinhardtii, SDS-PAGE Chlamydomonas reinhardtii
More the enzyme is found as large aggregates with different molecular masses in culture medium or under low salt concentration, MW 120000, 180000, 220000 and higher Chlamydomonas reinhardtii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Chlamydomonas reinhardtii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 5 min Chlamydomonas reinhardtii
50
-
 inactivation Chlamydomonas reinhardtii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Chlamydomonas reinhardtii