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Literature summary for 3.4.24.36 extracted from

  • Bouvier, J.; Schneider, P.; Etges, R.; Bordier, C.
    Peptide substrate specificity of the membrane-bound metalloprotease of Leishmania (1990), Biochemistry, 29, 10113-10119.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information possible autocatalytic mechanism for activation Leishmania sp.

Inhibitors

Inhibitors Comment Organism Structure
Benzyloxycarbonyl-Tyr-Leu-NHOH i.e. hydroxamate-derivatized dipeptide, strong, azocasein as substrate Leishmania sp.
additional information no inhibition by carboxymethyl derivative of Phe-Leu or hydroxamate derivatives of Leu or Tyr Leishmania sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
20
-
Leu-Ile-Ala-Tyr-Leu-Lys-Lys-Ala-Thr
-
Leishmania sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Leishmania sp. 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zinc zinc containing metalloproteinase Leishmania sp.

Organism

Organism UniProt Comment Textmining
Leishmania sp.
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
promastigote
-
Leishmania sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Arg-Ser-Val-Val-Arg-Asp-Val-Asn + H2O i.e. synthetic peptide derived from leishmanolysin Leishmania sp. Ala-Arg-Ser-Val + Val-Arg-Asp-Val-Asn
-
?
Ala-Arg-Ser-Val-Val-Arg-Asp-Val-Asn + H2O covers the last 4 amino acids of the prosequence and the first 5 amino acids of the native Leishmanolysin, cleavage site: Val-Val Leishmania sp. Ala-Arg-Ser-Val + Val-Arg-Asp-Val-Asn
-
?
azocasein + H2O
-
Leishmania sp. low molecular weight acid-soluble azopeptides
-
?
Glucagon + H2O major cleavage sites: Tyr10-Ser11, Asp15-Ser16, minor site: Trp25-Leu26 Leishmania sp. ?
-
?
Leu-Ile-Ala-Tyr-Leu-Lys-Lys-Ala-Thr + H2O from horse cytochrome Leishmania sp. Leu-Ile-Ala-Tyr + ?
-
?
Leu-Ile-Ala-Tyr-Leu-Lys-Lys-Ala-Thr + H2O model peptide cytochrome c(94-102)L, cleavage site: Tyr97-Leu98 Leishmania sp. Leu-Ile-Ala-Tyr + ?
-
?
Leu-Ile-Ala-Tyr-Ser-Lys-Lys-Ala-Thr + H2O from horse cytochrome Leishmania sp. ?
-
?
Leu-Ile-Ala-Tyr-Ser-Lys-Lys-Ala-Thr + H2O model peptide cytochrome c(94-102)S, cleavage site: Tyr97-Ser98 Leishmania sp. ?
-
?
additional information substrate specificity Leishmania sp. ?
-
?
additional information preferentially cleaves at amino side when hydrophobic residues are in P1'-site, basic amino acids in P2' and P3' and commonly Tyr at P1 Leishmania sp. ?
-
?
Oxidized insulin B-chain + H2O major cleavage site: Gly8-Ser9, minor site: Tyr16-Leu17 Leishmania sp. ?
-
?
Peptide 170-182 from CW-3 HLA antigen + H2O major cleavage site: Lys75-Asn76, minor site: Tyr73-Leu74 Leishmania sp. ?
-
?
Peptide 83-94 from beta-chain of HLA DO + H2O cleavage site: Arg84-Leu85 Leishmania sp. ?
-
?
Peptide 97-105 from Leishmanolysin + H2O
-
Leishmania sp. ?
-
?
Peptides from horse cytochrome c + H2O peptide 81-104 cleavage sites: Gly84-Ile85 and Tyr97-Leu98 Leishmania sp. ?
-
?
Peptides from horse cytochrome c + H2O i.e. peptides 1-25, 39-53 and 81-104 Leishmania sp. ?
-
?

Synonyms

Synonyms Comment Organism
Promastigote surface protease
-
Leishmania sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Leishmania sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8.5 Leu-Ile-Ala-Tyr-Leu-Lys-Lys-Ala-Thr as substrate Leishmania sp.
7.5 10 azocasein as substrate Leishmania sp.

pH Range

pH Minimum pH Maximum Comment Organism
4.7 11.7 about half-maximal activity at pH 4.7 and 11.7, azocasein as substrate Leishmania sp.
5.3 9.5 about half-maximal activity at pH 5.3 and 9.5, Leu-Ile-Ala-Tyr-Leu-Lys-Lys-Ala-Thr as substrate Leishmania sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 11 at least 30 min stable Leishmania sp.