Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | inactivation by dialysis against 1,10-phenanthroline, reactivation of apoenzyme by Zn2+, partially by Cu2+, Co2+, Cd2+ or Ni2+, not by Hg2+, Ca2+, Fe2+, Pb2+, Ba2+, Sn2+, Mg2+, Mn2+, Fe3+ or Ag+; strong, 1 mM, Zn2+ reverses, bovine serum albumin as substrate | Leishmania amazonensis | |
2,2'-bipyridyl | strong, Zn2+ reverses, bovine serum albumin as substrate | Leishmania amazonensis | |
2-mercaptoethylamine | Zn2+ reverses, bovine serum albumin as substrate | Leishmania amazonensis | |
8-hydroxyquinoline | Zn2+ reverses, bovine serum albumin as substrate | Leishmania amazonensis | |
EDTA | strong, Zn2+ reverses, bovine serum albumin as substrate | Leishmania amazonensis | |
EGTA | strong, Zn2+ reverses, bovine serum albumin as substrate | Leishmania amazonensis | |
thioglycolic acid | Zn2+ reverses, bovine serum albumin as substrate | Leishmania amazonensis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell surface | major surface protein | Leishmania amazonensis | 9986 | - |
cell surface | major surface protein | Leishmania mexicana pifanoi | 9986 | - |
membrane | - |
Leishmania amazonensis | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zinc | zinc containing metalloproteinase | Leishmania amazonensis | |
Zinc | zinc containing metalloproteinase | Leishmania mexicana pifanoi | |
Zinc | amino acid sequence between 161-173 and 304-308 shows strong homology to zinc-binding sites with the histidine ligands and the glutamate ligand of other metalloproteinases | Leishmania amazonensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Leishmania amazonensis | protects parasite membrane from cytosolic damage during their survival, differentiation and multiplication in the phagolysosomes of macrophages | ? | - |
? | |
additional information | Leishmania mexicana pifanoi | protects parasite membrane from cytosolic damage during their survival, differentiation and multiplication in the phagolysosomes of macrophages | ? | - |
? | |
additional information | Leishmania amazonensis LV78 | protects parasite membrane from cytosolic damage during their survival, differentiation and multiplication in the phagolysosomes of macrophages | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania amazonensis | - |
LV78 | - |
Leishmania amazonensis LV78 | - |
LV78 | - |
Leishmania mexicana pifanoi | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Leishmania amazonensis |
glycoprotein | - |
Leishmania mexicana pifanoi |
Purification (Comment) | Organism |
---|---|
promastigotes, monoclonal affinity purification | Leishmania amazonensis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
amastigote | enzymes from promastigotes and amastigotes are not identical but immunologically related | Leishmania amazonensis | - |
amastigote | enzymes from promastigotes and amastigotes are not identical but immunologically related | Leishmania mexicana pifanoi | - |
amastigote | axenic amastigotes from Leishmania mexicana pifanoi grown under macrophage-free conditions | Leishmania mexicana pifanoi | - |
promastigote | enzymes from promastigotes and amastigotes are not identical but immunologically related | Leishmania amazonensis | - |
promastigote | enzymes from promastigotes and amastigotes are not identical but immunologically related | Leishmania mexicana pifanoi | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Bovine serum albumin + H2O | radioiodinated | Leishmania amazonensis | ? | - |
? | |
Bovine serum albumin + H2O | radioiodinated | Leishmania mexicana pifanoi | ? | - |
? | |
Bovine serum albumin + H2O | radioiodinated | Leishmania amazonensis LV78 | ? | - |
? | |
Fibrinogen + H2O | - |
Leishmania amazonensis | ? | - |
? | |
Fibrinogen + H2O | - |
Leishmania amazonensis LV78 | ? | - |
? | |
Hemoglobin + H2O | - |
Leishmania amazonensis | ? | - |
? | |
Hemoglobin + H2O | - |
Leishmania mexicana pifanoi | ? | - |
? | |
Hemoglobin + H2O | - |
Leishmania amazonensis LV78 | ? | - |
? | |
additional information | non-specific endopeptidase | Leishmania amazonensis | ? | - |
? | |
additional information | protects parasite membrane from cytosolic damage during their survival, differentiation and multiplication in the phagolysosomes of macrophages | Leishmania amazonensis | ? | - |
? | |
additional information | protects parasite membrane from cytosolic damage during their survival, differentiation and multiplication in the phagolysosomes of macrophages | Leishmania mexicana pifanoi | ? | - |
? | |
additional information | non-specific endopeptidase | Leishmania amazonensis LV78 | ? | - |
? | |
additional information | protects parasite membrane from cytosolic damage during their survival, differentiation and multiplication in the phagolysosomes of macrophages | Leishmania amazonensis LV78 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Leishmania amazonensis |
37 | - |
assay at | Leishmania mexicana pifanoi |
60 | - |
- |
Leishmania amazonensis |
60 | - |
- |
Leishmania mexicana pifanoi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
promastigote enzyme | Leishmania amazonensis |