Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.24.35 extracted from

  • Mohseni, S.; Moghadam, T.T.; Dabirmanesh, B.; Khajeh, K.
    Expression, purification, refolding and invitro recovery of active full length recombinant human gelatinase MMP-9 in Escherichia coli (2016), Protein Expr. Purif., 126, 42-48 .
    View publication on PubMed
Search for more literature for 3.4.24.35

Cloned(Commentary)

Cloned (Comment) Organism
expression in the form of inclusion bodies in Escherichia coli BL21, using pET21a vector Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
EDTA 2 mM, severely inhibits enzyme activity due to chelation of metal ions Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ calcium and zinc dependent metalloproteinase Homo sapiens
Zn2+ calcium and zinc dependent metalloproteinase Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P14780
-
-

Purification (Commentary)

Purification (Comment) Organism
purification, refolding and in vitro recovery of active full length recombinant human gelatinase MMP-9 Homo sapiens

Renatured (Commentary)

Renatured (Comment) Organism
the effect of pH (in the range of 4.5-9.5) is investigated on the refolding of recombinant human MMP-9 by urea gradient method. Maximum recovery is obtained at pH 7.8 (with NaCl and Tris-HCl buffer) Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gelatin + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
MMP-9
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 gelatinolytic activity Homo sapiens