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Literature summary for 3.4.24.35 extracted from

  • Bigg, H.F.; Rowan, A.D.; Barker, M.D.; Cawston, T.E.
    Activity of matrix metalloproteinase-9 against native collagen types I and III (2007), FEBS J., 274, 1246-1255.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of MMP-9 in Spodoptera frugiperda Sf9 cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Homo sapiens
EDTA
-
Homo sapiens
TIMP-2
-
Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Homo sapiens
Zn2+
-
Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
86000
-
x * 92000, pro-MMP-9, SDS-PAGE, x * 86000, activated MMP-9, SDS-PAGE Homo sapiens
92000
-
x * 92000, pro-MMP-9, SDS-PAGE, x * 86000, activated MMP-9, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Collagen type I + H2O Homo sapiens
-
?
-
?
Collagen type III + H2O Homo sapiens
-
?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant MMP-9 from Spodoptera frugiperda Sf9 cells Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Collagen type I + H2O
-
Homo sapiens ?
-
?
Collagen type I + H2O soluble, native collagen type I, zymography Homo sapiens ?
-
?
Collagen type III + H2O
-
Homo sapiens ?
-
?
Collagen type III + H2O MMP-9 cleaves native, triple helical type III collagen to generate a 3/4 fragment, zymography Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
? x * 92000, pro-MMP-9, SDS-PAGE, x * 86000, activated MMP-9, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
matrix metalloproteinase-9
-
Homo sapiens
MMP-9
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 37 assay at Homo sapiens