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Literature summary for 3.4.24.34 extracted from

  • Bian, F.; Wang, C.; Tukler-Henriksson, J.; Pflugfelder, S.C.; Camodeca, C.; Nuti, E.; Rossello, A.; Li, D.Q.; de Paiva, C.S.
    MMP-8 is critical for dexamethasone therapy in alkali-burned corneas under dry eye conditions (2016), J. Cell. Physiol., 231, 2506-2516 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.34

Application

Application Comment Organism
medicine MMP-8 is critical for dexamethasone therapy in alkali-burned corneas under dry eye conditions. The anti-inflammatory effects of Dex are mediated through increased MMP-8 expression Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
gene MMP8, quantitative real-time PCR enzyme expression analysis Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information topical dexamethasone (Dex)-treated MMP-8 knockout mice in the corneal alkali burn murine model show reduced corneal clarity, increased expression of inflammatory mediators (IL-6, CXCL1, and MMP-1 mRNA) and increased neutrophil infiltration at 2D and 5D compared to Dex-treated wild-type mice. The accumulation of neutrophils is reduced in wounded corneas of MMP-8 knockout mice subjected to the corneal alkali burn murine (CM) model Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
2-((4'-morpholino-[1,1'-biphenyl]-4-yl) sulfonyl) benzoic acid
-
 Mus musculus
benzyl 2-((4'-morpholino-[1,1'-biphenyl]-4-yl) sulfonyl) benzoate
-
 Mus musculus
benzyl 2-((4-bromophenyl) sulfonyl) benzoate
-
 Mus musculus
additional information inhibitor syntheses, overview Mus musculus
N-hydroxy-2-((4'-morpholino-[1,1'-biphenyl]-4-yl) sulfonyl) benzamide CAM12, the MMP-8 inhibitor CAM12 causes the same phenotype compared to MMP-8 knockout mice Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Mus musculus
Zn2+ a zinc metalloproteinase Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus O70138
-
-
Mus musculus C57BL/6 O70138
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification pro-MMP-8 is activated by trypsin cleavage Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
cornea
-
 Mus musculus
-
epithelium corneal Mus musculus
-
eye
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Mca-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys(Dnp)-NH2 + H2O
-
 Mus musculus ?
-
 ?
Mca-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys(Dnp)-NH2 + H2O
-
 Mus musculus C57BL/6 ?
-
 ?
Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 + H2O
-
 Mus musculus ?
-
 ?
Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 + H2O
-
 Mus musculus C57BL/6 ?
-
 ?

Synonyms

Synonyms Comment Organism
MMP-8
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Mus musculus

Expression

Organism Comment Expression
Mus musculus dexamethasone (Dex) significantly increases MMP-8 mRNA transcripts in a concomitant dry eye and corneal alkali burn murine model up

General Information

General Information Comment Organism
additional information MMP-8 is critical for dexamethasone therapy in alkali-burned corneas under dry eye conditions. The anti-inflammatory effects of Dex are mediated through increased MMP-8 expression Mus musculus
physiological function MMP-8 is a collagen cleaving enzyme, produced by neutrophils and epithelial cells under inflammatory conditions, has been implicated in numerous tissue remodeling processes Mus musculus