Cloned (Comment) | Organism |
---|---|
expressed in Streptomyces lividans | Streptomyces cacaoi |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutations in the hydrophobic region or in the proline at the boundary of the hydrophobic core-cleavage region delay export of the enzyme and reduce total secretion by a factor of 2-10 | Streptomyces cacaoi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 10 mM inactivates activity almost completely, addition of 1 mM Zn2+, Co2+ or Mn2+ can restore depressed activity to 100, 142 and 115% respectively | Streptomyces cacaoi | |
additional information | not inhibited by PMSF and iodoacetamide | Streptomyces cacaoi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces cacaoi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by acetone precipitation and Fractogel CM-650 chromatography | Streptomyces cacaoi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
azocasein + H2O | - |
Streptomyces cacaoi | ? | - |
? | |
casein + H2O | - |
Streptomyces cacaoi | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Streptomyces cacaoi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | - |
Streptomyces cacaoi |