KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics, overview | Bacillus thermoproteolyticus | |
0.00191 | - |
N,N'-diBoc-dityrosyl-(Phe-isoniazid)2 | pH 7.5, 37°C | Bacillus thermoproteolyticus | |
0.00395 | - |
N,N'-diBoc-dityrosyl-(Ile-isoniazid)2 | pH 7.5, 37°C | Bacillus thermoproteolyticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | required | Bacillus thermoproteolyticus | |
NaCl | required | Bacillus thermoproteolyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus thermoproteolyticus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | synthesis and evaluation of substrates useful for the selective and sensitive assay of thermolysin, overview | Bacillus thermoproteolyticus | ? | - |
? | |
N,N'-diBoc-dityrosyl-(Ile-isoniazid)2 + H2O | - |
Bacillus thermoproteolyticus | N,N'-diBoc-dityrosyl + 2 Ile-isoniazid | - |
? | |
N,N'-diBoc-dityrosyl-(Phe-isoniazid)2 + H2O | - |
Bacillus thermoproteolyticus | N,N'-diBoc-dityrosyl + 2 Phe-isoniazid | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus thermoproteolyticus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0463 | - |
N,N'-diBoc-dityrosyl-(Phe-isoniazid)2 | pH 7.5, 37°C | Bacillus thermoproteolyticus | |
0.238 | - |
N,N'-diBoc-dityrosyl-(Ile-isoniazid)2 | pH 7.5, 37°C | Bacillus thermoproteolyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Bacillus thermoproteolyticus |
General Information | Comment | Organism |
---|---|---|
physiological function | thermolysin family proteases are related to various diseases such as bacterial infections, cholera, gastritis and peptic ulcers, and gastric carcinoma | Bacillus thermoproteolyticus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
24.3 | - |
N,N'-diBoc-dityrosyl-(Phe-isoniazid)2 | pH 7.5, 37°C | Bacillus thermoproteolyticus | |
60.3 | - |
N,N'-diBoc-dityrosyl-(Ile-isoniazid)2 | pH 7.5, 37°C | Bacillus thermoproteolyticus |