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Literature summary for 3.4.24.27 extracted from

  • Kim, C.; Lee, D.; Lee, C.; Ahn, I.
    Dityrosine-based substrates for the selective and sensitive assay of thermolysin (2015), J. Ind. Eng. Chem., 21, 248-253.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics, overview Bacillus thermoproteolyticus
0.00191
-
N,N'-diBoc-dityrosyl-(Phe-isoniazid)2 pH 7.5, 37°C Bacillus thermoproteolyticus
0.00395
-
N,N'-diBoc-dityrosyl-(Ile-isoniazid)2 pH 7.5, 37°C Bacillus thermoproteolyticus

Metals/Ions

Metals/Ions Comment Organism Structure
CaCl2 required Bacillus thermoproteolyticus
NaCl required Bacillus thermoproteolyticus

Organism

Organism UniProt Comment Textmining
Bacillus thermoproteolyticus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information synthesis and evaluation of substrates useful for the selective and sensitive assay of thermolysin, overview Bacillus thermoproteolyticus ?
-
?
N,N'-diBoc-dityrosyl-(Ile-isoniazid)2 + H2O
-
Bacillus thermoproteolyticus N,N'-diBoc-dityrosyl + 2 Ile-isoniazid
-
?
N,N'-diBoc-dityrosyl-(Phe-isoniazid)2 + H2O
-
Bacillus thermoproteolyticus N,N'-diBoc-dityrosyl + 2 Phe-isoniazid
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Bacillus thermoproteolyticus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0463
-
N,N'-diBoc-dityrosyl-(Phe-isoniazid)2 pH 7.5, 37°C Bacillus thermoproteolyticus
0.238
-
N,N'-diBoc-dityrosyl-(Ile-isoniazid)2 pH 7.5, 37°C Bacillus thermoproteolyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Bacillus thermoproteolyticus

General Information

General Information Comment Organism
physiological function thermolysin family proteases are related to various diseases such as bacterial infections, cholera, gastritis and peptic ulcers, and gastric carcinoma Bacillus thermoproteolyticus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
24.3
-
N,N'-diBoc-dityrosyl-(Phe-isoniazid)2 pH 7.5, 37°C Bacillus thermoproteolyticus
60.3
-
N,N'-diBoc-dityrosyl-(Ile-isoniazid)2 pH 7.5, 37°C Bacillus thermoproteolyticus