Crystallization (Comment) | Organism |
---|---|
native thermolysin in complex with 3-methylaspirin, 3-methylaspirin ethyl ester, and 3-methylaspirin cyclopropyl ester, sitting drop vapor diffusion method, using water as reservoir solution, at 18°C | Bacillus thermoproteolyticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-(acetyloxy)-3-chlorobenzoic acid | - |
Bacillus thermoproteolyticus | |
2-ethyl-3-hydroxyquinazolin-4(3H)-one | - |
Bacillus thermoproteolyticus | |
3-hydroxy-2-isopropylquinazolin-4(3H)-one | - |
Bacillus thermoproteolyticus | |
3-hydroxy-2-methylquinazolin-4(3H)-one | - |
Bacillus thermoproteolyticus | |
3-methylaspirin | - |
Bacillus thermoproteolyticus | |
additional information | not inhibited by 3-hydroxy-2-methylthieno[3,2-d]pyrimidine-4(3H)-one and 3-(acetylamino)-4-methylthiophene-2-carboxylic acid | Bacillus thermoproteolyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus thermoproteolyticus | P00800 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-N-(4-[4'-N',N'-(dimethylamino)phenylazo]-benzoyl-L-serinyl-L-phenylalanylamido)-N''-ethylaminonaphthalene-5-sulfonic acid + H2O | - |
Bacillus thermoproteolyticus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TLN | - |
Bacillus thermoproteolyticus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.128 | - |
3-hydroxy-2-isopropylquinazolin-4(3H)-one | in 100 mM Tris/HCl, pH 7.5, 2 mM CaCl2, 4% (v/v) DMSO, at 25°C | Bacillus thermoproteolyticus | |
0.315 | - |
2-ethyl-3-hydroxyquinazolin-4(3H)-one | in 100 mM Tris/HCl, pH 7.5, 2 mM CaCl2, 4% (v/v) DMSO, at 25°C | Bacillus thermoproteolyticus | |
0.57 | - |
2-(acetyloxy)-3-chlorobenzoic acid | in 100 mM Tris/HCl, pH 7.5, 2 mM CaCl2, 4% (v/v) DMSO, at 25°C | Bacillus thermoproteolyticus | |
0.93 | - |
3-hydroxy-2-methylquinazolin-4(3H)-one | in 100 mM Tris/HCl, pH 7.5, 2 mM CaCl2, 4% (v/v) DMSO, at 25°C | Bacillus thermoproteolyticus | |
1.7 | - |
3-methylaspirin | in 100 mM Tris/HCl, pH 7.5, 2 mM CaCl2, 4% (v/v) DMSO, at 25°C | Bacillus thermoproteolyticus |