Crystallization (Comment) | Organism |
---|---|
resiudes H142, H146, and E166 coordinate the catalytic zinc, while E143 and H231 are required for the catalytic activity | Bacillus thermoproteolyticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-beta-D-arabinofuranosyl-N4-lauroylcytosine | competitive | Bacillus thermoproteolyticus | |
2-hydroxy-N-(4-methyl-2-nitrophenyl)-3-nitrobenzamide | competitive | Bacillus thermoproteolyticus | |
5-bromo-N-(4-bromophenyl)-2-hydroxy-3-nitro-benzamide | competitive | Bacillus thermoproteolyticus | |
additional information | identification of inhibitors by structure based virtual screening and study of binding modes by docking | Bacillus thermoproteolyticus | |
N-(2,3-dimethylphenyl)-2-hydroxy-3-nitro-benzamide | competitive | Bacillus thermoproteolyticus | |
N-(2,4-dimethylphenyl)-2-hydroxy-3-nitro-benzamide | competitive | Bacillus thermoproteolyticus | |
N-(2,5-dimethylphenyl)-2-hydroxy-3-nitrobenzamide | competitive | Bacillus thermoproteolyticus | |
N-(2-chloro-4-nitrophenyl)-2-hydroxy-3-nitro-benzamide | competitive | Bacillus thermoproteolyticus | |
N-(2-chloro-6-methylphenyl)-2-hydroxy-3-nitrobenzamide | competitive | Bacillus thermoproteolyticus | |
N-(2-ethylphenyl)-2-hydroxy-3-nitrobenzamide | competitive | Bacillus thermoproteolyticus | |
N-(5-chloro-2-methoxyphenyl)-2-hydroxy-3-nitro-benzamide | competitive | Bacillus thermoproteolyticus | |
oxyquinoline | - |
Bacillus thermoproteolyticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | residues H142, H146, and E166 coordinate the catalytic zinc | Bacillus thermoproteolyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus thermoproteolyticus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-beta-D-arabinofuranosyl-N4-lauroylcytosine + H2O | - |
Bacillus thermoproteolyticus | ? | - |
? | |
2-hydroxy-N-(4-methyl-2-nitrophenyl)-3-nitrobenzamide + H2O | - |
Bacillus thermoproteolyticus | ? | - |
? | |
5-bromo-N-(4-bromophenyl)-2-hydroxy-3-nitro-benzamide + H2O | - |
Bacillus thermoproteolyticus | ? | - |
? | |
N-(2,3-dimethylphenyl)-2-hydroxy-3-nitro-benzamide + H2O | - |
Bacillus thermoproteolyticus | ? | - |
? | |
N-(2,4-dimethylphenyl)-2-hydroxy-3-nitro-benzamide + H2O | - |
Bacillus thermoproteolyticus | ? | - |
? | |
N-(2,5-dimethylphenyl)-2-hydroxy-3-nitrobenzamide + H2O | - |
Bacillus thermoproteolyticus | ? | - |
? | |
N-(2-chloro-4-nitrophenyl)-2-hydroxy-3-nitro-benzamide + H2O | - |
Bacillus thermoproteolyticus | ? | - |
? | |
N-(2-chloro-6-methylphenyl)-2-hydroxy-3-nitrobenzamide + H2O | - |
Bacillus thermoproteolyticus | ? | - |
? | |
N-(2-ethylphenyl)-2-hydroxy-3-nitrobenzamide + H2O | - |
Bacillus thermoproteolyticus | ? | - |
? | |
N-(5-chloro-2-methoxyphenyl)-2-hydroxy-3-nitro-benzamide + H2O | - |
Bacillus thermoproteolyticus | ? | - |
? | |
N-[3-(2-furyl)acryloyl]glycyl-L-leucinamide + H2O | - |
Bacillus thermoproteolyticus | ? | - |
? | |
oxyquinoline + H2O | - |
Bacillus thermoproteolyticus | ? | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000000064 | - |
1-beta-D-arabinofuranosyl-N4-lauroylcytosine | 25°C, pH 7.5 | Bacillus thermoproteolyticus | |
0.047 | - |
N-(2-chloro-6-methylphenyl)-2-hydroxy-3-nitrobenzamide | 25°C, pH 7.5 | Bacillus thermoproteolyticus | |
0.214 | - |
oxyquinoline | 25°C, pH 7.5 | Bacillus thermoproteolyticus | |
1.45 | - |
N-(2,5-dimethylphenyl)-2-hydroxy-3-nitrobenzamide | 25°C, pH 7.5 | Bacillus thermoproteolyticus | |
2.06 | - |
2-hydroxy-N-(4-methyl-2-nitrophenyl)-3-nitrobenzamide | 25°C, pH 7.5 | Bacillus thermoproteolyticus | |
2.95 | - |
N-(2-ethylphenyl)-2-hydroxy-3-nitrobenzamide | 25°C, pH 7.5 | Bacillus thermoproteolyticus | |
4.81 | - |
5-bromo-N-(4-bromophenyl)-2-hydroxy-3-nitro-benzamide | 25°C, pH 7.5 | Bacillus thermoproteolyticus | |
8.14 | - |
N-(5-chloro-2-methoxyphenyl)-2-hydroxy-3-nitro-benzamide | 25°C, pH 7.5 | Bacillus thermoproteolyticus | |
161 | - |
N-(2,4-dimethylphenyl)-2-hydroxy-3-nitro-benzamide | 25°C, pH 7.5 | Bacillus thermoproteolyticus | |
294 | - |
N-(2,3-dimethylphenyl)-2-hydroxy-3-nitro-benzamide | 25°C, pH 7.5 | Bacillus thermoproteolyticus | |
697 | - |
N-(2-chloro-4-nitrophenyl)-2-hydroxy-3-nitro-benzamide | 25°C, pH 7.5 | Bacillus thermoproteolyticus |