Protein Variants | Comment | Organism |
---|---|---|
A113D | complete loss of activity | Bacillus thermoproteolyticus |
A113E | complete loss of activity | Bacillus thermoproteolyticus |
A113H | complete loss of activity | Bacillus thermoproteolyticus |
A113K | complete loss of activity | Bacillus thermoproteolyticus |
A113R | complete loss of activity | Bacillus thermoproteolyticus |
D150A | 131% residual activity with casein, 81% residual activity with substrate N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | Bacillus thermoproteolyticus |
D150E | 128% residual activity with casein, 228% residual activity with substrate N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | Bacillus thermoproteolyticus |
D150H | 105% residual activity with casein, 37% residual activity with substrate N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide. Thermal inactivation at 80°C is greatly suppressed | Bacillus thermoproteolyticus |
D150K | 51% residual activity with casein, no residual activity with substrate N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | Bacillus thermoproteolyticus |
D150R | 44% residual activity with casein, no residual activity with substrate N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | Bacillus thermoproteolyticus |
D150W | 81% residual activity with casein, 60% residual activity with substrate N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide. Thermal inactivation at 80°C is greatly suppressed | Bacillus thermoproteolyticus |
D170A | complete loss of activity | Bacillus thermoproteolyticus |
D170E | complete loss of activity | Bacillus thermoproteolyticus |
D170H | complete loss of activity | Bacillus thermoproteolyticus |
D170K | complete loss of activity | Bacillus thermoproteolyticus |
D170R | complete loss of activity | Bacillus thermoproteolyticus |
F114A | 28% residual activity with casein, 8% residual activity with substrate N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | Bacillus thermoproteolyticus |
F114D | complete loss of activity | Bacillus thermoproteolyticus |
F114E | complete loss of activity | Bacillus thermoproteolyticus |
F114H | 18% residual activity with casein, 20% residual activity with substrate N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | Bacillus thermoproteolyticus |
F114K | complete loss of activity | Bacillus thermoproteolyticus |
F114R | complete loss of activity | Bacillus thermoproteolyticus |
G162A | complete loss of activity | Bacillus thermoproteolyticus |
G162D | complete loss of activity | Bacillus thermoproteolyticus |
G162E | complete loss of activity | Bacillus thermoproteolyticus |
G162H | complete loss of activity | Bacillus thermoproteolyticus |
G162K | complete loss of activity | Bacillus thermoproteolyticus |
G162R | complete loss of activity | Bacillus thermoproteolyticus |
I168A | 69% residual activity with casein, 125% residual activity with substrate N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide. Thermal inactivation at 80°C is greatly suppressed | Bacillus thermoproteolyticus |
I168D | complete loss of activity | Bacillus thermoproteolyticus |
I168E | complete loss of activity | Bacillus thermoproteolyticus |
I168H | 13% residual activity with casein, 35% residual activity with substrate N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide. Thermal inactivation at 80°C is greatly suppressed | Bacillus thermoproteolyticus |
I168K | complete loss of activity | Bacillus thermoproteolyticus |
I168R | complete loss of activity | Bacillus thermoproteolyticus |
N227A | 72% residual activity with casein , 28% residual activity with substrate N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide. Thermal inactivation at 80°C is greatly suppressed | Bacillus thermoproteolyticus |
N227D | 11% residual activity with casein, no residual activity with substrate N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | Bacillus thermoproteolyticus |
N227E | 36% residual activity with casein, no residual activity with substrate N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | Bacillus thermoproteolyticus |
N227H | 19% residual activity with casein, 19% residual activity with substrate N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide. Thermal inactivation at 80°C is greatly suppressed | Bacillus thermoproteolyticus |
N227K | 29% residual activity with casein, no residual activity with substrate N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | Bacillus thermoproteolyticus |
N227R | 55% residual activity with casein, no residual activity with substrate N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | Bacillus thermoproteolyticus |
S169A | 112% residual activity with casein, 64% residual activity with substrate N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | Bacillus thermoproteolyticus |
S169D | complete loss of activity | Bacillus thermoproteolyticus |
S169E | complete loss of activity | Bacillus thermoproteolyticus |
S169H | complete loss of activity | Bacillus thermoproteolyticus |
S169K | complete loss of activity | Bacillus thermoproteolyticus |
S169R | complete loss of activity | Bacillus thermoproteolyticus |
S234A | 88% residual activity with casein, 17% residual activity with substrate N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide. Thermal inactivation at 80°C is greatly suppressed | Bacillus thermoproteolyticus |
S234D | 5% residual activity with casein, no residual activity with substrate N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | Bacillus thermoproteolyticus |
S234E | 4% residual activity with casein, 7% residual activity with substrate N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | Bacillus thermoproteolyticus |
S234H | 32% residual activity with casein, no residual activity with substrate N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | Bacillus thermoproteolyticus |
S234K | complete loss of activity | Bacillus thermoproteolyticus |
S234R | complete loss of activity | Bacillus thermoproteolyticus |
V230A | 17% residual activity with casein, no residual activity with substrate N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | Bacillus thermoproteolyticus |
V230D | complete loss of activity | Bacillus thermoproteolyticus |
V230E | complete loss of activity | Bacillus thermoproteolyticus |
V230H | complete loss of activity | Bacillus thermoproteolyticus |
V230K | 3% residual activity with casein, no residual activity with substrate N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | Bacillus thermoproteolyticus |
V230R | 6% residual activity with casein, 12% residual activity with substrate N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | Bacillus thermoproteolyticus |
W115A | complete loss of activity | Bacillus thermoproteolyticus |
W115D | complete loss of activity | Bacillus thermoproteolyticus |
W115E | complete loss of activity | Bacillus thermoproteolyticus |
W115H | complete loss of activity | Bacillus thermoproteolyticus |
W115K | complete loss of activity | Bacillus thermoproteolyticus |
W115R | complete loss of activity | Bacillus thermoproteolyticus |
Y157A | 11% residual activity with casein, no residual activity with substrate N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | Bacillus thermoproteolyticus |
Y157D | 1% residual activity with casein, no residual activity with substrate N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | Bacillus thermoproteolyticus |
Y157E | 13% residual activity with casein, no residual activity with substrate N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | Bacillus thermoproteolyticus |
Y157H | 24% residual activity with casein, no residual activity with substrate N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | Bacillus thermoproteolyticus |
Y157K | 7% residual activity with casein, no residual activity with substrate N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide | Bacillus thermoproteolyticus |
Y157R | complete loss of activity | Bacillus thermoproteolyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus thermoproteolyticus | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.34 | - |
mutant F114H, substrate casein, 25°C, pH 7.5 | Bacillus thermoproteolyticus |
0.42 | - |
mutant I168H, substrate casein, 25°C, pH 7.5 | Bacillus thermoproteolyticus |
0.42 | - |
mutant S234A, substrate casein, 25°C, pH 7.5 | Bacillus thermoproteolyticus |
0.55 | - |
mutant N227A, substrate casein, 25°C, pH 7.5 | Bacillus thermoproteolyticus |
0.62 | - |
mutant D150W, substrate casein, 25°C, pH 7.5 | Bacillus thermoproteolyticus |
0.68 | - |
mutant I168A, substrate casein, 25°C, pH 7.5 | Bacillus thermoproteolyticus |
0.84 | - |
mutant D150H, substrate casein, 25°C, pH 7.5 | Bacillus thermoproteolyticus |
0.89 | - |
mutant D150A, substrate casein, 25°C, pH 7.5 | Bacillus thermoproteolyticus |
0.89 | - |
mutant S169A, substrate casein, 25°C, pH 7.5 | Bacillus thermoproteolyticus |
0.93 | - |
mutant D150E, substrate casein, 25°C, pH 7.5 | Bacillus thermoproteolyticus |
1.08 | - |
wild-type, substrate casein, 25°C, pH 7.5 | Bacillus thermoproteolyticus |
1.14 | - |
mutant F114A, substrate casein, 25°C, pH 7.5 | Bacillus thermoproteolyticus |
1.18 | - |
mutant N227H, substrate casein, 25°C, pH 7.5 | Bacillus thermoproteolyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
casein + H2O | - |
Bacillus thermoproteolyticus | ? | - |
? | |
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide + H2O | - |
Bacillus thermoproteolyticus | ? | - |
? |