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Literature summary for 3.4.24.27 extracted from
- Molecular mechanism of the inhibitory effect of cobalt ion on thermolysin activity and the suppressive effect of calcium ion on the cobalt ion-dependent inactivation of thermolysin (2007), J. Biochem., 141, 879-888.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | competitive, 72% inhibition at 18 mM, partial protection or reverse effect by Ca2+ at 0.5 mM, Co2+ show partly Ca2+-sensitive and partly Ca2+-insensitive inhibition, molecular mechanism, Co21 accelerates the autolysis, overview | Bacillus thermoproteolyticus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Bacillus thermoproteolyticus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | four ions per enzyme molecule required for stability | Bacillus thermoproteolyticus | |
| Zn2+ | one ion per enzyme molecule required for activity | Bacillus thermoproteolyticus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus thermoproteolyticus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | thermolysin performs Co2+-stimulable autolysis | Bacillus thermoproteolyticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | thermolysin performs Co2+-stimulable autolysis | Bacillus thermoproteolyticus | ? | - |
? | |
| N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide + H2O | i.e. FAGLA | Bacillus thermoproteolyticus | ? | - |
? | |
| N-[3-(2-furyl)acryloyl]-L-leucine-L-alanine amide + H2O | i.e. FALAA | Bacillus thermoproteolyticus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Bacillus thermoproteolyticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Bacillus thermoproteolyticus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | inhibition kinetics with Co2+ and Ca2+, overview | Bacillus thermoproteolyticus |
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Release 2023.1 (January 2023)
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