Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.24.27 extracted from

  • Hashida, Y.; Inouye, K.
    Kinetic analysis of the activation-and-inhibition dual effects of cobalt ion on thermolysin activity (2007), J. Biochem., 141, 843-853.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Co2+ increases the activity 3-4fold at up to 2 mM, but inhibits at 2-18 mM, activation-and-inhibition dual effects of Co2+ ion are analysed kinetically Bacillus thermoproteolyticus
Zn2+
-
Bacillus thermoproteolyticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Bacillus thermoproteolyticus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ four ions per enzyme molecule required for stability Bacillus thermoproteolyticus
Co2+ increases the activity 3-4fold at up to 2 mM, but inhibits at 2-18 mM, activation-and-inhibition dual effects of Co2+ ion are analysed kinetically, Co2+-dependent activation is inhibited competitively by Zn2+ ion at 0.0001-0.001 mM Bacillus thermoproteolyticus
additional information thermolysin activity as well as its stability is remarkably enhanced by high concentration of neutral salts consisting of Na+, K+, Cl-, and Br- in the synthesis and hydrolysis of N-carbobenzoxy-L-aspertyl-L-phenylalanine methyl ester and hydrolysis of N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide Bacillus thermoproteolyticus
Zn2+ one ion per enzyme molecule required for activity Bacillus thermoproteolyticus

Organism

Organism UniProt Comment Textmining
Bacillus thermoproteolyticus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
Bacillus thermoproteolyticus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-carbobenzoxy-L-Asp-L-Phe methyl ester + H2O
-
Bacillus thermoproteolyticus ?
-
?
N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide + H2O i.e. FAGLA Bacillus thermoproteolyticus ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Bacillus thermoproteolyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Bacillus thermoproteolyticus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information detailed inhibition kinetics with Co2+ and Zn2+, overview Bacillus thermoproteolyticus