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Literature summary for 3.4.24.27 extracted from

  • Inouye, K.; Minoda, M.; Takita, T.; Sakurama, H.; Hashida, Y.; Kusano, M.; Yasukawa, K.
    Extracellular production of recombinant thermolysin expressed in Escherichia coli, and its purification and enzymatic characterization (2006), Protein Expr. Purif., 46, 248-255.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
N-carbobenzoxy-L-Asp-L-Phe methyl ester pH 7.5, 25°C, recombinant enzyme Bacillus thermoproteolyticus
0.52
-
N-carbobenzoxy-L-Asp-L-Phe methyl ester pH 7.5, 25°C, native enzyme Bacillus thermoproteolyticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34600
-
x * 34600, SDS-PAGE Bacillus thermoproteolyticus

Organism

Organism UniProt Comment Textmining
Bacillus thermoproteolyticus
-
expressed in Escherichia coli
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification synthesis as a pre-proprotein Bacillus thermoproteolyticus

Purification (Commentary)

Purification (Comment) Organism
expression as single polypeptide pre-proenzyme in Escherichia coli, secretion into medium as mature enzyme Bacillus thermoproteolyticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9500
-
pH 7.5, 25°C Bacillus thermoproteolyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-carbobenzoxy-L-Asp-L-Phe-methyl ester + H2O
-
Bacillus thermoproteolyticus N-carbobenzoxy-L-Asp + L-Phe-methyl ester
-
?
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide + H2O
-
Bacillus thermoproteolyticus ?
-
?

Subunits

Subunits Comment Organism
? x * 34600, SDS-PAGE Bacillus thermoproteolyticus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.8
-
N-carbobenzoxy-L-Asp-L-Phe methyl ester pH 7.5, 25°C, recombinant enzyme Bacillus thermoproteolyticus
4.3
-
N-carbobenzoxy-L-Asp-L-Phe methyl ester pH 7.5, 25°C, native enzyme Bacillus thermoproteolyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
Bacillus thermoproteolyticus