Literature summary for 3.4.24.27 extracted from

Inagaki, T.; Tadasa, K.; Kayahara, H.
Modification effects of organic solvents on microenvironment of the enzyme in thermolysin-catalyzed peptide synthesis of N-(benzyloxycarbonyl)-L-phenylalanyl-L-phenylalanine methyl ester (1994), Biosci. Biotechnol. Biochem., 58, 1439-1442.

No PubMed abstract available

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Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	direct effect of organic solvents on the microenvironment of the enzyme largely depends on the molecular structure of the solvent	Bacillus thermoproteolyticus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
39.2	-	N-(benzyloxycarbonyl)-L-Phe	-	Bacillus thermoproteolyticus
250	-	L-Phe methyl ester	pH 5.5 or 6.0	Bacillus thermoproteolyticus
300	-	L-Phe methyl ester	-	Bacillus thermoproteolyticus

Organism

Organism	UniProt	Comment	Textmining
Bacillus thermoproteolyticus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-(Benzyloxycarbonyl)-L-Phe + L-Phe methyl ester	-	Bacillus thermoproteolyticus	N-(Benzyloxycarbonyl)-L-Phe-L-Phe methyl ester + H2O	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.69	-	N-(benzyloxycarbonyl)-L-Phe	-	Bacillus thermoproteolyticus

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Release 2023.1 (January 2023)