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Literature summary for 3.4.24.24 extracted from

  • Wang, C.; Zhan, C.; Cai, Q.; Du, C.; Liu, G.; Su, W.; Cao, M.
    Expression and characterization of common carp (Cyprinus carpio) matrix metalloproteinase-2 and its activity against type I collagen (2014), J. Biotechnol., 177, 45-52.
    View publication on PubMed
Search for more literature for 3.4.24.24

Cloned(Commentary)

Cloned (Comment) Organism
gene MMP-2 , cloning from skeletal muscle, DNA and amino acid sequence determination and analysis, sequence comparissons, expression of recombinant His-tagged MMP-2 in truncated form in Escherichia coli inclusion bodies Cyprinus carpio

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline complete inhibition Cyprinus carpio
EDTA complete inhibition Cyprinus carpio
EGTA complete inhibition Cyprinus carpio
additional information PMSF, benzamidine, E-64, and pepstatin A are poor inhibitors Cyprinus carpio

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ activates Cyprinus carpio
Ca2+ divalent metal ion Ca2+ is absolutely necessary for the gelatinolytic activity, suggesting a calcium-dependent metalloproteinase Cyprinus carpio
additional information no or poor activation by Zn2+, Mn2+, Mg2+, Cu2+, Fe2+, or Co2+ Cyprinus carpio

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Gelatin + H2O Cyprinus carpio
-
 ?
-
 ?
Type I collagen + H2O Cyprinus carpio degradation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Cyprinus carpio M4VLM9 gene MMP-2
-

Purification (Commentary)

Purification (Comment) Organism
renaturation of recombinant MMP-2 in truncated form from Escherichia coli strain BL21(DE3) inclusion bodies after nickel affinity chromatography Cyprinus carpio

Renatured (Commentary)

Renatured (Comment) Organism
renaturation of recombinant MMP-2 in truncated form from Escherichia coli strain BL21(DE3) inclusion bodies after nickel affinity chromatography Cyprinus carpio

Source Tissue

Source Tissue Comment Organism Textmining
skeletal muscle
-
 Cyprinus carpio
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gelatin + H2O
-
 Cyprinus carpio ?
-
 ?
Type I collagen + H2O
-
 Cyprinus carpio ?
-
 ?
Type I collagen + H2O degradation Cyprinus carpio ?
-
 ?

Synonyms

Synonyms Comment Organism
matrix metalloproteinase-2
-
 Cyprinus carpio
MMP-2
-
 Cyprinus carpio

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 recombinant enzyme Cyprinus carpio

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 45 activity range Cyprinus carpio

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 recombinant enzyme Cyprinus carpio

pH Range

pH Minimum pH Maximum Comment Organism
7 9 activity range Cyprinus carpio

General Information

General Information Comment Organism
additional information crucial role of the fibronectin-like domain in enzymatic activities, it is involved in substrate binding Cyprinus carpio