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Literature summary for 3.4.24.24 extracted from

  • Akers, W.J.; Xu, B.; Lee, H.; Sudlow, G.; Fields, G.B.; Achilefu, S.; Edwards, W.B.
    Detection of MMP-2 and MMP-9 activity in vivo with a triple-helical peptide optical probe (2012), Bioconjug. Chem., 23, 656-663.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
ilomastat a pan-MMP inhibitor, strong inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic analysis of LS276-THP hydrolysis by MMP-2 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
fibrosarcoma cell tumor periphery Homo sapiens
-
HT-1080 cell tumour cells developed in mice, high expression level Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
LS276-THP + H2O development and evaluation of an activatable NIR fluorescent probe LS276-THP for in vivo detection of cancer-related matrix metalloproteinase activity based on a triplehelical peptide substrate with high specificity for MMP-2 and MMP-9 relative to other members of the MMP family, overview. Triple-helical peptides are suitable for highly specific in vivo detection of tumor-related MMP-2 and MMP-9 activity Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
MMP-2
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0000005
-
pH 7.4, 37°C Homo sapiens ilomastat

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
30000
-
LS276-THP pH 7.4, 37°C Homo sapiens