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Literature summary for 3.4.24.23 extracted from

  • Muta, Y.; Yasui, N.; Matsumiya, Y.; Kubo, M.; Inouye, K.
    Expression in Escherichia coli, refolding, and purification of the recombinant mature form of human matrix metalloproteinase 7 (MMP-7) (2010), Biosci. Biotechnol. Biochem., 74, 2515-2517.
    View publication on PubMed
Search for more literature for 3.4.24.23

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain BL21(DE3) in inclusion bodies Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ the inactive pre-proenzyme is cleaved to the pro-peptide, which is then activated when the propeptide residue Cys70 binds to the active site Zn2+ Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the inactive pre-proenzyme is cleaved to the pro-peptide, which is then activated when the propeptide residue Cys70 binds to the active site Zn2+ Homo sapiens

Renatured (Commentary)

Renatured (Comment) Organism
recombinant MMP-7 from Escherichia coli strain BL21(DE3) inclusion bodies, solubilization and refolding at 1 M L-arginine Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(7-methoxycoumarin-4-yl)-acetyl-Pro-Leu-Gly-Leu-[N-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg amide + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
Matrix metalloproteinase 7
-
 Homo sapiens
MMP-7
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens

General Information

General Information Comment Organism
additional information the inactive pre-proenzyme is cleaved to the pro-peptide, which is then activated when the propeptide residue Cys70 binds to the active site Zn2+ Homo sapiens