Literature summary for 3.4.24.23 extracted from

Ra, H.J.; Harju-Baker, S.; Zhang, F.; Linhardt, R.J.; Wilson, C.L.; Parks, W.C.
Control of promatrilysin (MMP7) activation and substrate-specific activity by sulfated glycosaminoglycans (2009), J. Biol. Chem., 284, 27924-27932.

Search for more literature for 3.4.24.23

Cloned(Commentary)

Cloned (Comment)	Organism
expression of wild-type and mutant matrilysins in Escherichia coli strain BL21 in inclusion bodies	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
E-cadherin + H2O	Homo sapiens	matrilysin cleaves E-cadherin in its juxtamembrane stalk releasing the entire ectodomain	modified E-cadherin + E-cadherin ectodomain	-	?
fibronactin + H2O	Homo sapiens	-	fibronectin peptide fragments	MWs of 30 to 175 kDa	?
pro-alpha-defensin-1 + H2O	Homo sapiens	i.e. procryptdins	alpha-defensin-1 + alpha-defensin-1 propeptide	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
proteolytic modification	promatrilysin shows autolytic activation, promatrilysin activation is regulated by sulfated glycosaminoglycans, overview. Presence of chondroitin-4,6-sulfate markedly accelerates activation of promatrilysin. Chondroitin-4,6-sulfate and heparin also markedly accelerates the ability of active wild-type matrilysin to cleave inactive promatrilysin	Homo sapiens

Purification (Commentary)

Purification (Comment)	Organism
recombinant wild-type and mutant matrilysins, after solubilization from Escherichia coli strain BL21 inclusion bodies, by cation exchange chromatography and gel filtration	Homo sapiens

Renatured (Commentary)

Renatured (Comment)	Organism
recombinant wild-type and mutant matrilysins from Escherichia coli strain BL21 inclusion bodies dialyzed in 8 M urea containing 50 mM Tris, pH 7.4, 50 mM NaCl, 10 mM CaCl2, 0.1 mM zinc acetate, 0.05% Brij-35, 0.02% NaN3, and 0.01% Triton X-100, followed by gel filtration	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(7-methoxy-coumarin-4-yl)acetyl-Ala-Pro-Lys-2,4-dinitrophenol + H2O	-	Homo sapiens	?	-	?
E-cadherin + H2O	matrilysin cleaves E-cadherin in its juxtamembrane stalk releasing the entire ectodomain	Homo sapiens	modified E-cadherin + E-cadherin ectodomain	-	?
fibronactin + H2O	-	Homo sapiens	fibronectin peptide fragments	MWs of 30 to 175 kDa	?
pro-alpha-defensin-1 + H2O	i.e. procryptdins	Homo sapiens	alpha-defensin-1 + alpha-defensin-1 propeptide	-	?

Synonyms

Synonyms	Comment	Organism
MMP7	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Homo sapiens

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Release 2023.1 (January 2023)