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Literature summary for 3.4.24.20 extracted from

  • Dohmae, N.; Hayashi, K.; Miki, K.; Tsumuraya, Y.; Hashimoto, Y.
    Purification and characterization of intracellular proteinases in Pleurotus ostreatus fruiting bodies (1995), Biosci. Biotechnol. Biochem., 59, 2074-2080.
    View publication on PubMed
Search for more literature for 3.4.24.20

General Stability

General Stability Organism
Stable to 5 h at 37°C in 0.1 M acetate buffer, pH 5, containing 2 M urea, 1 M guanidine-HCl or 0.01% SDS, inactivation at higher concentrations of urea, guanidine-HCl or SDS Pleurotus ostreatus

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Pleurotus ostreatus
2,2'-bipyridine 1 mM, weak Pleurotus ostreatus
2-mercaptoethanol not Pleurotus ostreatus
Ag2+ 1 mM, strong Pleurotus ostreatus
antipain not Pleurotus ostreatus
Cu2+ 1 mM, caseinolytic activity; strong Pleurotus ostreatus
dithiothreitol caseinolytic activity; weak Pleurotus ostreatus
EDTA 50 mM, weak at 1 mM; fully restorable by Zn2+, Mn2+, Co2+ Pleurotus ostreatus
Guanidine-HCl 2 M and above, not at 1 M Pleurotus ostreatus
Hg2+ 1 mM; strong, caseinolytic activity Pleurotus ostreatus
additional information L-cysteine; no inhibition (of caseinolytic activity) by chymostatin, iodoacetate, leupeptin; PMSF Pleurotus ostreatus
N-ethylmaleimide weak, caseinolytic activity Pleurotus ostreatus
p-chloromercuribenzoate weak, caseinolytic activity Pleurotus ostreatus
phosphoramidon not Pleurotus ostreatus
SDS 0.1% and above, not at 0.01% Pleurotus ostreatus
tosyl-Lys chloromethyl ketone weak, caseinolytic Pleurotus ostreatus
Urea 5 M, not at 2 M Pleurotus ostreatus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Pleurotus ostreatus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zinc requirement, 1 atom per molecule of native enzyme Pleurotus ostreatus
Zinc atomic absorption spectroscopy Pleurotus ostreatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
 1 * 18000, Pleurotus ostreatus, SDS-PAGE Pleurotus ostreatus

Organism

Organism UniProt Comment Textmining
Pleurotus ostreatus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pleurotus ostreatus

Source Tissue

Source Tissue Comment Organism Textmining
fruit body
-
 Pleurotus ostreatus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Pleurotus ostreatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azocasein + H2O
-
 Pleurotus ostreatus ?
-
 ?
Bovine insulin B-chain + H2O cleavage site: Pro29-Lys30, yielding 2 fragments: dipeptide Lys-Ala and substrate devoid of 2 C-terminal amino acids Pleurotus ostreatus ?
-
 ?
Equine heart cytochrome c + H2O
-
 Pleurotus ostreatus ?
-
 ?
Polyarginine + H2O
-
 Pleurotus ostreatus ?
-
 ?
Polylysine + H2O best substrate Pleurotus ostreatus ?
-
 ?
protamine sulfate + H2O
-
 Pleurotus ostreatus ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 18000, Pleurotus ostreatus, SDS-PAGE Pleurotus ostreatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Pleurotus ostreatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 15 min, 10% loss of caseinolytic activity Pleurotus ostreatus
70
-
 15 min, inactivation Pleurotus ostreatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 pI: 8.35 Pleurotus ostreatus
5.6
-
 azocoll or azocasein as substrate Pleurotus ostreatus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 9 18 h, 10% loss of caseinolytic activity at 4°C Pleurotus ostreatus