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Literature summary for 3.4.24.19 extracted from
- Data comparing the kinetics of procollagen type I processing by bone morphogenetic protein 1 (BMP-1) with and without procollagen C-proteinase enhancer 1 (PCPE-1) (2016), Data Brief, 9, 883-887 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| procollagen C-proteinase enhancer 1 | PCPE-1, CUB (Complement C1r/C1s, Uegf, BMP-1) and netrin-like (NTR) domains fragments of PCPE-1 are generated by limited proteolysis with trypsin and isolated by heparin affinity chromatography | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant enzyme expression in insect cells using the baculovirus transfection system | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic constants using Lineweaver-Burk plots and Michaelis-Menten equation | Homo sapiens | |
| 0.00009 | - |
Procollagen type I | pH and temperature not specified in the publication, recombinant enzyme, in presence of PCPE-1 CUB1/CUB2 domains | Homo sapiens |  |
| 0.0001 | - |
Procollagen type I | pH and temperature not specified in the publication, recombinant enzyme, in presence of PCPE-1 | Homo sapiens | |
| 0.00019 | - |
Procollagen type I | pH and temperature not specified in the publication, recombinant enzyme, in presence of PCPE-1 NTR domain | Homo sapiens | |
| 0.00039 | - |
Procollagen type I | pH and temperature not specified in the publication, recombinant enzyme | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| procollagen type I + H2O | Homo sapiens | - |
collagen type I + collagen type I propeptide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P13497 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| procollagen type I + H2O | - |
Homo sapiens | collagen type I + collagen type I propeptide | - |
? | |
| procollagen type I + H2O | usage of chicken embryo fibroblasts tendon procollagen type I labeled biosynthetically with H3-tryptophan | Homo sapiens | collagen type I + collagen type I propeptide | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BMP-1 | - |
Homo sapiens |
| bone morphogenetic protein 1 | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.013 | - |
Procollagen type I | pH and temperature not specified in the publication, recombinant enzyme, in presence of PCPE-1 NTR domain | Homo sapiens | |
| 0.016 | - |
Procollagen type I | pH and temperature not specified in the publication, recombinant enzyme | Homo sapiens | |
| 0.053 | - |
Procollagen type I | pH and temperature not specified in the publication, recombinant enzyme, in presence of PCPE-1 CUB1/CUB2 domains | Homo sapiens | |
| 0.059 | - |
Procollagen type I | pH and temperature not specified in the publication, recombinant enzyme, in presence of PCPE-1 | Homo sapiens | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the C-terminal processing of procollagen type I by bone morphogenetic protein 1, BMP-1, the major procollagen C-proteinase, is a reaction stimulated by the connective tissue glycoprotein procollagen C-proteinase enhancer 1 (PCPE-1), kinetic constants | Homo sapiens |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 41.03 | - |
Procollagen type I | pH and temperature not specified in the publication, recombinant enzyme | Homo sapiens | |
| 68.42 | - |
Procollagen type I | pH and temperature not specified in the publication, recombinant enzyme, in presence of PCPE-1 NTR domain | Homo sapiens | |
| 588.9 | - |
Procollagen type I | pH and temperature not specified in the publication, recombinant enzyme, in presence of PCPE-1 CUB1/CUB2 domains | Homo sapiens | |
| 590 | - |
Procollagen type I | pH and temperature not specified in the publication, recombinant enzyme, in presence of PCPE-1 | Homo sapiens | |
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