Cloned (Comment) | Organism |
---|---|
gene MEP1A, recombinant expression of N-terminally Strep-tagged meprin alpha in Drosophila melanogaster S2 cells, which are stably transfected with pMT/BiP/V5-C-hMep alpha22-600 | Homo sapiens |
gene MEP1B, recombinant expression of meprin alpha in Pichia pastoris strain X-33 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
actinonin | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Homo sapiens | |
additional information | - |
additional information | enzyme kinetic data for meprin alpha suggests hyperbolic v/S characteristics | Homo sapiens | |
0.024 | - |
KKGYVADAP-4-nitroanilide | pH 7.5, 30°C, recombinant enzyme | Homo sapiens | |
0.024 | - |
KKGYVADAP-4-nitroanilide | pH 7.4, 30°C, recombinant enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Homo sapiens | |
Zn2+ | required, a zinc-metalloprotease | Homo sapiens | |
Zn2+ | zinc-dependent metalloendoprotease | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | meprin beta preferentially cleaves substrates with acidic amino acids in P1'-position | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q16819 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the recombinant His6-tagged enzyme is activated by cleavage through trypsin | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally Strep-tagged meprin alpha22-600 from Drosophila melanogaster S2 cells by hydrophobic interaction chromatography in an expanded bed system, followed by Strep-tactin affinity chromatography, activation of the enzyme by trypsin, and gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
KKGYVADAP-4-nitroanilide + H2O | - |
Homo sapiens | KKGYVA + DAP-4-nitroanilide | - |
? | |
additional information | meprin beta preferentially cleaves substrates with acidic amino acids in P1'-position | Homo sapiens | ? | - |
? | |
additional information | the cleavage of a meprin alpha substrate leads to generation of the prolyl tripeptidyl aminopeptidase (PtP, EC 3.4.14.12) substrate, and the activity of PtP results in release of a chromophore or fluorophore, coupled assay method evaluation, overview | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MEP1A | - |
Homo sapiens |
meprin A subunit alpha | UniProt | Homo sapiens |
meprin alpha | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
KKGYVADAP-4-nitroanilide | pH 7.5, 30°C, recombinant enzyme | Homo sapiens | |
1 | - |
KKGYVADAP-4-nitroanilide | pH 7.4, 30°C, recombinant enzyme | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
7.5 | - |
- |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | the astacin proteases meprin alpha and meprin beta are zinc-dependent metalloproteases of the metzincin superfamily | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
41.7 | - |
KKGYVADAP-4-nitroanilide | pH 7.5, 30°C, recombinant enzyme | Homo sapiens | |
41.7 | - |
KKGYVADAP-4-nitroanilide | pH 7.4, 30°C, recombinant enzyme | Homo sapiens |