Cloned (Comment) | Organism |
---|---|
stable expression of HA-tagged alpha-and beta-subunits in HEK-293 cells and secretion to the culture medium | Homo sapiens |
stable expression of HA-tagged alpha-subunit in HEK-293 cells and secretion to the culture medium | Homo sapiens |
stable expression of HA-tagged beta-subunit in HEK-293 cells and secretion to the culture medium | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
TAPI-1 | a hydroxamate inhibitor; a hydroxamate inhibitor | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | ADAM10 is the major sheddase responsible for the release of membrane-associated meprin A | Homo sapiens | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Homo sapiens | Q16819 | subunit alpha | - |
Homo sapiens | Q16820 | subunit beta | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | ADAM10 is the major sheddase responsible for the release of membrane-associated meprin A | Homo sapiens |
proteolytic modification | ADAM10 is the major sheddase responsible for the release of membrane-associated meprin A. ADAM10 is the protease responsible for the shedding of meprin beta from the meprin beta and meprin alphabeta transfectants, determination by inhibiotr experiments. Both constitutive as well as PMA- and ionomycin-induced shedding of meprin A in the primary renal tubular epithelial cells is markedly prevented by the hydroxamate inhibitor TAPI-1 and the ADAM10-specific inhibitor GI254023X | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant extracellular HA-tagged alpha-subunit from HEK-293 cell culture medium by ultrafiltration | Homo sapiens |
recombinant extracellular HA-tagged beta-subunit from HEK-293 cell culture medium by ultrafiltration | Homo sapiens |
recombinant extracellular HA-tagged enzyme dimer from HEK-293 cell culture medium by ultrafiltration | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Homo sapiens | - |
renal proximal tubule | meprin A, composed of alpha and beta subunits, is the major form of meprin present in the apical membranes of renal proximal tubules | Homo sapiens | - |
Subunits | Comment | Organism |
---|---|---|
heterodimer | alphabeta | Homo sapiens |