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Literature summary for 3.4.24.18 extracted from

  • Bao, J.; Yura, R.E.; Matters, G.L.; Bradley, S.G.; Shi, P.; Tian, F.; Bond, J.S.
    Meprin A impairs epithelial barrier function, enhances monocyte migration, and cleaves the tight junction protein occludin (2013), Am. J. Physiol. Renal Physiol., 305, F714-F726.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of homomeric enzyme in HEK-293 cells Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mus musculus no activity with claudin-4 in MDCK cells ?
-
?
additional information Canis lupus familiaris no activity with claudin-4 in MDCK cells ?
-
?
additional information Mus musculus C57BL/6 no activity with claudin-4 in MDCK cells ?
-
?
additional information Canis lupus familiaris Madin-Darby no activity with claudin-4 in MDCK cells ?
-
?
occludin + H2O Mus musculus in MDCK cells ?
-
?
occludin + H2O Canis lupus familiaris in MDCK cells and in micelles ?
-
?
occludin + H2O Mus musculus C57BL/6 in MDCK cells ?
-
?
occludin + H2O Canis lupus familiaris Madin-Darby in MDCK cells and in micelles ?
-
?

Organism

Organism UniProt Comment Textmining
Canis lupus familiaris
-
-
-
Canis lupus familiaris Madin-Darby
-
-
-
Mus musculus
-
-
-
Mus musculus C57BL/6
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Canis lupus familiaris
-
MDCK cell
-
Canis lupus familiaris
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with claudin-4 in MDCK cells Mus musculus ?
-
?
additional information no activity with claudin-4 in MDCK cells Canis lupus familiaris ?
-
?
additional information no activity with claudin-4 in MDCK cells Mus musculus C57BL/6 ?
-
?
additional information no activity with claudin-4 in MDCK cells Canis lupus familiaris Madin-Darby ?
-
?
occludin + H2O in MDCK cells Mus musculus ?
-
?
occludin + H2O in MDCK cells and in micelles Canis lupus familiaris ?
-
?
occludin + H2O recombinant MBP-conjugated human substrate and recombinant enzyme, cleavage products of extracellular loop 1 and loop 2 are determined by mass spectrometry, overview. The cleavage site is between Gly100 and Ser101 is on the first extracellular loop of occludin Canis lupus familiaris ?
-
?
occludin + H2O recombinant substrate in micelles and recombinant enzyme, cleavage products of extracellular loop 1 and loop 2 are determined by mass spectrometry, overview. The cleavage site is between Gly100 and Ser101 is on the first extracellular loop of occludin Mus musculus ?
-
?
occludin + H2O in MDCK cells Mus musculus C57BL/6 ?
-
?
occludin + H2O recombinant substrate in micelles and recombinant enzyme, cleavage products of extracellular loop 1 and loop 2 are determined by mass spectrometry, overview. The cleavage site is between Gly100 and Ser101 is on the first extracellular loop of occludin Mus musculus C57BL/6 ?
-
?
occludin + H2O in MDCK cells and in micelles Canis lupus familiaris Madin-Darby ?
-
?
occludin + H2O recombinant MBP-conjugated human substrate and recombinant enzyme, cleavage products of extracellular loop 1 and loop 2 are determined by mass spectrometry, overview. The cleavage site is between Gly100 and Ser101 is on the first extracellular loop of occludin Canis lupus familiaris Madin-Darby ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Mus musculus
37
-
assay at Canis lupus familiaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
assay at Mus musculus
6.8
-
assay at Canis lupus familiaris

General Information

General Information Comment Organism
malfunction monocytes from meprin knockout mice on a C57BL/6 background are less able to migrate through an MDCK cell monolayer than monocytes from their wild-type counterparts Mus musculus
metabolism meprin A is more effective than meprin B, EC 3.4.24.63, in impairing MDCK epithelial barrier function Canis lupus familiaris
physiological function the activity of meprin A enables monocytes to migrate through an epithelial barrier more readily allowing inflammatory molecules such as cytokines and monocytes to gain access to sites of injury. Meprin A impairs epithelial barrier function, enhances monocyte migration, and cleaves the tight junction protein occludin Mus musculus
physiological function the activity of meprin A enables monocytes to migrate through an epithelial barrier more readily allowing inflammatory molecules such as cytokines and monocytes to gain access to sites of injury. Meprin A impairs epithelial barrier function, enhances monocyte migration, and cleaves the tight junction protein occludin Canis lupus familiaris