Cloned (Comment) | Organism |
---|---|
recombinant expression of homomeric enzyme in HEK-293 cells | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mus musculus | no activity with claudin-4 in MDCK cells | ? | - |
? | |
additional information | Canis lupus familiaris | no activity with claudin-4 in MDCK cells | ? | - |
? | |
additional information | Mus musculus C57BL/6 | no activity with claudin-4 in MDCK cells | ? | - |
? | |
additional information | Canis lupus familiaris Madin-Darby | no activity with claudin-4 in MDCK cells | ? | - |
? | |
occludin + H2O | Mus musculus | in MDCK cells | ? | - |
? | |
occludin + H2O | Canis lupus familiaris | in MDCK cells and in micelles | ? | - |
? | |
occludin + H2O | Mus musculus C57BL/6 | in MDCK cells | ? | - |
? | |
occludin + H2O | Canis lupus familiaris Madin-Darby | in MDCK cells and in micelles | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Canis lupus familiaris | - |
- |
- |
Canis lupus familiaris Madin-Darby | - |
- |
- |
Mus musculus | - |
- |
- |
Mus musculus C57BL/6 | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Canis lupus familiaris | - |
MDCK cell | - |
Canis lupus familiaris | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with claudin-4 in MDCK cells | Mus musculus | ? | - |
? | |
additional information | no activity with claudin-4 in MDCK cells | Canis lupus familiaris | ? | - |
? | |
additional information | no activity with claudin-4 in MDCK cells | Mus musculus C57BL/6 | ? | - |
? | |
additional information | no activity with claudin-4 in MDCK cells | Canis lupus familiaris Madin-Darby | ? | - |
? | |
occludin + H2O | in MDCK cells | Mus musculus | ? | - |
? | |
occludin + H2O | in MDCK cells and in micelles | Canis lupus familiaris | ? | - |
? | |
occludin + H2O | recombinant MBP-conjugated human substrate and recombinant enzyme, cleavage products of extracellular loop 1 and loop 2 are determined by mass spectrometry, overview. The cleavage site is between Gly100 and Ser101 is on the first extracellular loop of occludin | Canis lupus familiaris | ? | - |
? | |
occludin + H2O | recombinant substrate in micelles and recombinant enzyme, cleavage products of extracellular loop 1 and loop 2 are determined by mass spectrometry, overview. The cleavage site is between Gly100 and Ser101 is on the first extracellular loop of occludin | Mus musculus | ? | - |
? | |
occludin + H2O | in MDCK cells | Mus musculus C57BL/6 | ? | - |
? | |
occludin + H2O | recombinant substrate in micelles and recombinant enzyme, cleavage products of extracellular loop 1 and loop 2 are determined by mass spectrometry, overview. The cleavage site is between Gly100 and Ser101 is on the first extracellular loop of occludin | Mus musculus C57BL/6 | ? | - |
? | |
occludin + H2O | in MDCK cells and in micelles | Canis lupus familiaris Madin-Darby | ? | - |
? | |
occludin + H2O | recombinant MBP-conjugated human substrate and recombinant enzyme, cleavage products of extracellular loop 1 and loop 2 are determined by mass spectrometry, overview. The cleavage site is between Gly100 and Ser101 is on the first extracellular loop of occludin | Canis lupus familiaris Madin-Darby | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
37 | - |
assay at | Canis lupus familiaris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Mus musculus |
6.8 | - |
assay at | Canis lupus familiaris |
General Information | Comment | Organism |
---|---|---|
malfunction | monocytes from meprin knockout mice on a C57BL/6 background are less able to migrate through an MDCK cell monolayer than monocytes from their wild-type counterparts | Mus musculus |
metabolism | meprin A is more effective than meprin B, EC 3.4.24.63, in impairing MDCK epithelial barrier function | Canis lupus familiaris |
physiological function | the activity of meprin A enables monocytes to migrate through an epithelial barrier more readily allowing inflammatory molecules such as cytokines and monocytes to gain access to sites of injury. Meprin A impairs epithelial barrier function, enhances monocyte migration, and cleaves the tight junction protein occludin | Mus musculus |
physiological function | the activity of meprin A enables monocytes to migrate through an epithelial barrier more readily allowing inflammatory molecules such as cytokines and monocytes to gain access to sites of injury. Meprin A impairs epithelial barrier function, enhances monocyte migration, and cleaves the tight junction protein occludin | Canis lupus familiaris |