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Literature summary for 3.4.24.18 extracted from

  • Villa, J.P.; Bertenshaw, G.P.; Bond, J.S.
    Critical Amino Acids in the Active Site of Meprin Metalloproteinases for Substrate and Peptide Bond Specificity (2003), J. Biol. Chem., 278, 42545-42550.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.18

Activating Compound

Activating Compound Comment Organism Structure
Trypsin
-
 Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in human embryonic kidney 293 cells ATCC 1573 CRL Mus musculus

Protein Variants

Protein Variants Comment Organism
F161R site-directed mutagenesis Mus musculus
P228K site-directed mutagenesis Mus musculus
Y199K site-directed mutagenesis Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 cholecystokinin 8-sulfate pH 7.5, 37°C, mutant Y199K Mus musculus
0.23
-
 cholecystokinin 8-sulfate pH 7.5, 37°C, mutant F161R Mus musculus
0.29
-
 cholecystokinin 8-sulfate pH 7.5, 37°C, mutant P228K Mus musculus
0.44
-
 gastrin 17 pH 7.5, 37°C, mutant Y199K Mus musculus
0.49
-
 cholecystokinin 8-sulfate pH 7.5, 37°C Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ zinc-metalloendopeptidase Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
 mouse
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azocasein + H2O
-
 Mus musculus ?
-
 ?
cholecystokinin 8-sulfate + H2O
-
 Mus musculus ?
-
 ?
gastrin 17 + H2O mutant Y199K Mus musculus ?
-
 ?
additional information orcokinin and gastrin 17 are no substrates Mus musculus ?
-
 ?

Synonyms

Synonyms Comment Organism
meprin metalloproteinase
-
 Mus musculus
mouse meprin alpha
-
 Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.8
-
 cholecystokinin 8-sulfate pH 7.5, 37°C, mutant P228K Mus musculus
8.6
-
 cholecystokinin 8-sulfate pH 7.5, 37°C Mus musculus
10.8
-
 gastrin 17 pH 7.5, 37°C, mutant Y199K Mus musculus
13.4
-
 cholecystokinin 8-sulfate pH 7.5, 37°C, mutant F161R Mus musculus
14.2
-
 cholecystokinin 8-sulfate pH 7.5, 37°C, mutant Y199K Mus musculus