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Literature summary for 3.4.24.18 extracted from
- Zinc ligands in an astacin family metalloprotease meprin A (2002), Biol. Chem., 383, 1167-1173.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| human embryonic kidney 293 cells stably transfected with cDNA | Mus musculus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H167A | site-directed mutagenesis | Mus musculus |
| Y226F | site-directed mutagenesis | Mus musculus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0121 | - |
bradykinin | pH 7.5, 37°C, mutant Y226F | Mus musculus |  |
| 0.0163 | - |
bradykinin | pH 7.5, 37°C, wild-type | Mus musculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| plasma membrane | membrane-bound | Mus musculus | 5886 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | 5 zinc ligands proposed to coordinate zinc at the active site | Mus musculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 90000 | - |
8 * 90000, SDS-PAGE | Mus musculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
mouse | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mus musculus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.9 | - |
mutant Y226F | Mus musculus |
| 20.8 | - |
wild-type | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-aminobenzoyl-RPPGFSPFRK-(dinitrophenyl)-G + H2O | fluorogenic bradykinin analog substrate | Mus musculus | ? | - |
? | |
| bradykinin + H2O | - |
Mus musculus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| octamer | 8 * 90000, SDS-PAGE | Mus musculus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 52 | - |
quite stable, less than 10% activity lost in 30 min | Mus musculus |
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Release 2023.1 (January 2023)
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