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Literature summary for 3.4.24.17 extracted from

  • Nagase, H.
    Human stromelysins 1 and 2 (1995), Methods Enzymol., 248, 449-470.
    View publication on PubMed

General Stability

General Stability Organism
Ca2+ is required to maintain the active conformation, full activity is detected at 1 mM Ca2+, at pH 7.5 and is retained at even higher concentrations of Ca2+, at lower concentrations the enzyme is autolyzed Homo sapiens
Ca2+ stabilizes Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Homo sapiens
1,10-phenanthroline
-
Oryctolagus cuniculus
Alpha-macroglobulin
-
Homo sapiens
cysteine
-
Homo sapiens
DTT
-
Homo sapiens
DTT
-
Oryctolagus cuniculus
EDTA
-
Bos taurus
EDTA
-
Homo sapiens
EDTA
-
Oryctolagus cuniculus
EDTA
-
Rattus norvegicus
EGTA
-
Homo sapiens
HONH-CO-CH2-CH(n-pentyl)-CO-Leu-Phe-NH2
-
Homo sapiens
Ovostatin
-
Homo sapiens
Phthaloyl-N-(CH2)4-P(O2-)-Ile-(2-naphthyl)-Ala-NH-CH3
-
Homo sapiens
Tissue inhibitor of metalloproteinases TIMP-1 and TIMP-2 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
(7-Methoxycoumarin-4-yl)acetyl-Arg-Pro-Lys-Pro-Val-Glu-norvaline-Trp-Arg-Lys(2,4-dinitrophenyl)-NH2
-
Homo sapiens
0.1
-
2,4-Dinitrophenyl-Pro-Tyr-Ala-Tyr-Trp-Met-Arg-NH2
-
Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ Ca2+ is required to maintain the active conformation of the enzyme, full activity is detected at 1 mM Ca2+, at pH 7.5 and is retained at even higher concentrations of Ca2+, at lower concentrations the enzyme is autolyzed Homo sapiens
Ca2+ Ca2+ is required to maintain the active conformation of the enzyme, full activity is detected at 1 mM Ca2+, at pH 7.5 and is retained at even higher concentrations of Ca2+, at lower concentrations the enzyme is autolyzed Rattus norvegicus
Ca2+ Ca2+ is required to maintain the active conformation of the enzyme, full activity is detected at 1 mM Ca2+, at pH 7.5 and is retained at even higher concentrations of Ca2+, at lower concentrations the enzyme is autolyzed Bos taurus
Ca2+ Ca2+ is required to maintain the active conformation of the enzyme, full activity is detected at 1 mM Ca2+, at pH 7.5 and is retained at even higher concentrations of Ca2+, at lower concentrations the enzyme is autolyzed Oryctolagus cuniculus
Ca2+ proenzyme contains 3 Ca2+ Homo sapiens
Ca2+ proenzyme contains 3 Ca2+ Rattus norvegicus
Ca2+ proenzyme contains 3 Ca2+ Bos taurus
Ca2+ proenzyme contains 3 Ca2+ Oryctolagus cuniculus
Zinc zinc metalloprotein, proenzyme contains two Zn2+, the Zn2+ at the active site is bound to His-201, His-205, and His-211 and Cys-75 in the propeptide Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
stromelysin 1 is synthesized as a preproenzyme of 477 amino acids and is secreted from cells as a proenzyme of 460 amino acids which consist of the propeptide (82 amino acids), the catalytic domain (165 amino acids), the proline-rich region (25 amino acids), and the C-terminal domain (188 amino acids) Homo sapiens
additional information
-
stromelysin 1 is synthesized as a preproenzyme of 477 amino acids and is secreted from cells as a proenzyme of 460 amino acids which consist of the propeptide (82 amino acids), the catalytic domain (165 amino acids), the proline-rich region (25 amino acids), and the C-terminal domain (188 amino acids) Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Homo sapiens
-
-
-
Oryctolagus cuniculus
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
cartilage
-
Homo sapiens
-
cartilage
-
Rattus norvegicus
-
cartilage
-
Bos taurus
-
cartilage
-
Oryctolagus cuniculus
-
culture medium synovial fibroblasts Homo sapiens
-
culture medium synovial fibroblasts Rattus norvegicus
-
culture medium synovial fibroblasts Bos taurus
-
culture medium synovial fibroblasts Oryctolagus cuniculus
-
additional information the enzyme is not readily detected in normal cells in culture or tissue homogenates, but its synthesis is greatly enhanced in mesenchymal cells when they are treated with cytokines such as interleukin-1 and tumor necrosis factor alpha, growth factors, phorbol myristate, acetate and several other stimuli Homo sapiens
-
additional information the enzyme is not readily detected in normal cells in culture or tissue homogenates, but its synthesis is greatly enhanced in mesenchymal cells when they are treated with cytokines such as interleukin-1 and tumor necrosis factor alpha, growth factors, phorbol myristate, acetate and several other stimuli Rattus norvegicus
-
additional information the enzyme is not readily detected in normal cells in culture or tissue homogenates, but its synthesis is greatly enhanced in mesenchymal cells when they are treated with cytokines such as interleukin-1 and tumor necrosis factor alpha, growth factors, phorbol myristate, acetate and several other stimuli Bos taurus
-
additional information the enzyme is not readily detected in normal cells in culture or tissue homogenates, but its synthesis is greatly enhanced in mesenchymal cells when they are treated with cytokines such as interleukin-1 and tumor necrosis factor alpha, growth factors, phorbol myristate, acetate and several other stimuli Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
assay methods Homo sapiens
additional information
-
assay methods Rattus norvegicus
additional information
-
assay methods Bos taurus
additional information
-
assay methods Oryctolagus cuniculus

Storage Stability

Storage Stability Organism
-20°C, 50 mM Tris-HCl, pH 7.5, 0.15 M NaCl, 10 mM CaCl2, 0.02% w/v NaN3, 0.05% w/v Brij 35, stable for at least 1 year, prostromelysin 1 Homo sapiens
4°C, 50 mM Tris-HCl, pH 7.5, 0.15 M NaCl, 10 mM CaCl2, 0.02% w/v NaN3, 0.05% w/v Brij 35, stable for at least 4 months, prostromelysin 1 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(7-Methoxycoumarin-4-yl)acetyl-Arg-Pro-Lys-Pro-Val-Glu-norvaline-Trp-Arg-Lys(2,4-dinitrophenyl)-NH2 + H2O
-
Homo sapiens (7-Methoxycoumarin-4-yl)acetyl-Arg-Pro-Lys-Pro-Val-Glu + norvaline-Trp-Arg-Lys(2,4-dinitrophenyl)-NH2
-
?
(7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-N-3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl-Ala-Arg-NH2 + H2O
-
Homo sapiens (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly + Leu-N-3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl-Ala-Arg-NH2
-
?
2,4-Dinitrophenyl-Arg-Pro-Lys-Pro-Leu-Ala-norvaline-Trp-NH2 + H2O
-
Homo sapiens 2,4-Dinitrophenyl-Arg-Pro-Lys-Pro-Leu-Ala + norvaline-Trp-NH2
-
?
2,4-Dinitrophenyl-Pro-Leu-Gly-Leu-Trp-Ala-D-Arg-NH2 + H2O
-
Homo sapiens 2,4-Dinitrophenyl-Pro-Leu-Gly + Leu-Trp-Ala-D-Arg-NH2
-
?
2,4-Dinitrophenyl-Pro-Tyr-Ala-Tyr-Trp-Met-Arg-NH2 + H2O
-
Homo sapiens 2,4-Dinitrophenyl-Pro-Tyr-Ala + Tyr-Trp-Met-Arg-NH2
-
?
Aggrecan core protein + H2O
-
Homo sapiens ?
-
?
casein + H2O
-
Homo sapiens ?
-
?
casein + H2O
-
Oryctolagus cuniculus ?
-
?
Collagen type IV + H2O
-
Homo sapiens More
-
?
Collagen type IV + H2O
-
Oryctolagus cuniculus More
-
?
Elastin + H2O limited activity Homo sapiens ?
-
?
Elastin + H2O limited activity Rattus norvegicus ?
-
?
Elastin + H2O limited activity Bos taurus ?
-
?
Elastin + H2O limited activity Oryctolagus cuniculus ?
-
?
Fibronectin + H2O
-
Homo sapiens Intact fibronectin subunit MW 22000 + a disulfide-bonded COOH-terminal MW 20000 polypeptide
-
?
Fibronectin + H2O
-
Oryctolagus cuniculus Intact fibronectin subunit MW 22000 + a disulfide-bonded COOH-terminal MW 20000 polypeptide
-
?
Gelatin + H2O type 1 Homo sapiens ?
-
?
Gelatin + H2O type 1 Rattus norvegicus ?
-
?
Gelatin + H2O type 1 Bos taurus ?
-
?
Gelatin + H2O type 1 Oryctolagus cuniculus ?
-
?
additional information activates procollagenase Homo sapiens ?
-
?
additional information activates procollagenase Rattus norvegicus ?
-
?
additional information activates procollagenase Bos taurus ?
-
?
additional information activates procollagenase Oryctolagus cuniculus ?
-
?
additional information activates progelatinase B Homo sapiens ?
-
?
additional information activates progelatinase B Rattus norvegicus ?
-
?
additional information activates progelatinase B Bos taurus ?
-
?
additional information activates progelatinase B Oryctolagus cuniculus ?
-
?
additional information enzyme is secreted from the cells as an inactive zymogen Homo sapiens ?
-
?
additional information enzyme is secreted from the cells as an inactive zymogen Rattus norvegicus ?
-
?
additional information enzyme is secreted from the cells as an inactive zymogen Bos taurus ?
-
?
additional information enzyme is secreted from the cells as an inactive zymogen Oryctolagus cuniculus ?
-
?
additional information degrades a number of extracellular matrix components Homo sapiens ?
-
?
additional information degrades a number of extracellular matrix components Rattus norvegicus ?
-
?
additional information degrades a number of extracellular matrix components Bos taurus ?
-
?
additional information degrades a number of extracellular matrix components Oryctolagus cuniculus ?
-
?
additional information participates in activation of procollagenases and progelatinase B Homo sapiens ?
-
?
additional information participates in activation of procollagenases and progelatinase B Rattus norvegicus ?
-
?
additional information participates in activation of procollagenases and progelatinase B Bos taurus ?
-
?
additional information participates in activation of procollagenases and progelatinase B Oryctolagus cuniculus ?
-
?
additional information cleavage sites in natural proteins Homo sapiens ?
-
?
additional information cleavage sites in natural proteins Rattus norvegicus ?
-
?
additional information cleavage sites in natural proteins Bos taurus ?
-
?
additional information cleavage sites in natural proteins Oryctolagus cuniculus ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens
37
-
assay at Rattus norvegicus
37
-
assay at Bos taurus
37
-
assay at Oryctolagus cuniculus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
48 h, stable, prostromelysin Homo sapiens
80
-
15 min, 35% loss of activity Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.24
-
2,4-Dinitrophenyl-Pro-Tyr-Ala-Tyr-Trp-Met-Arg-NH2
-
Homo sapiens
5.4
-
(7-Methoxycoumarin-4-yl)acetyl-Arg-Pro-Lys-Pro-Val-Glu-norvaline-Trp-Arg-Lys(2,4-dinitrophenyl)-NH2
-
Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3 5.5 digestion of aggrecan core protein Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5
-
more than 80% loss of activity after 15 h Homo sapiens
5
-
about 50% loss of activity after 15 h Homo sapiens
5.5 9.5 stable in this range Homo sapiens
10
-
about 50% loss of activity after 15 h Homo sapiens
10.5
-
more than 80% loss of activity after 15 h Homo sapiens