Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | catalytic activity of recombinant Nln increases over time upon incubation in fresh mouse blood. The recombinant protein easily reaches the systemiccirculation remaining both catalytically active and physically intact | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli | Rattus norvegicus |
General Stability | Organism |
---|---|
up to two cycles of freezing and thawing of the purified rNln do not significantly affect the purified recombinant enzyme's specific activity | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Rattus norvegicus | |
Zn2+ | zinc metalloproteinase | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P42676 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography, ultrafiltration, and gel filtration to homogeneity, large scale production of rNln | Rattus norvegicus |
Storage Stability | Organism |
---|---|
a gradual decrease of the specific activity is observed in preparations kept longer than 6 months at -80°C | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
angiotensin I + H2O | - |
Rattus norvegicus | ? | - |
? | |
bradykinin + H2O | - |
Rattus norvegicus | ? | - |
? | |
neurotensin + H2O | - |
Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 75000, recombinant His6-tagged enzyme, SDS-PAGE | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
Nln | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Rattus norvegicus |