Literature summary for 3.4.24.15 extracted from

Akopyan, T.N.; Couedel, Y.; Orlowski, M.; Fournie-Zaluski, M.C.; Roques, B.P.
Proteolytic processing of farnesylated peptides: assay and partial purification from pig brain membranes of an endopeptidase which has the characteristics of E.C. 3.4.24.15 (1994), Biochem. Biophys. Res. Commun., 198, 787-794.

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Inhibitors

Inhibitors	Comment	Organism	Structure
1,10-phenanthroline	-	Sus scrofa
chymostatin	-	Sus scrofa
Cu2+	0.1 mM, 90% inhibition	Sus scrofa
dithiothreitol	-	Sus scrofa
EDTA	no inhibition	Sus scrofa
N-(phenylethylphosphonyl)-Gly-L-Pro-L-aminohexanoic acid	-	Sus scrofa
NEM	-	Sus scrofa
PCMB	-	Sus scrofa
Zn2+	0.1 mM ZnCl2, complete inhibition	Sus scrofa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
32.5	-	propionyl-Gly-Ser-Pro-(farnesyl-Cys)-Val-Leu-Met	-	Sus scrofa

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
microsome	-	Sus scrofa	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
70000	-	gel filtration	Sus scrofa

Organism

Organism	UniProt	Comment	Textmining
Sus scrofa	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Sus scrofa

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	-	Sus scrofa	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.00037	-	-	Sus scrofa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
propionyl-Gly-Ser-Pro-(farnesyl-Cys)-Val-Leu-Met	-	Sus scrofa	propionyl-Gly-Ser-Pro-(farnesyl-Cys) + Val-Leu-Met	-	?

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.2	7.5	-	Sus scrofa

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	8.5	pH 6.0: about 35% of maximal activity, pH 8.5: about 45% of maximal activity	Sus scrofa

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Release 2023.1 (January 2023)