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Literature summary for 3.4.24.14 extracted from

  • Hojima, Y.; McKenzie, J.A.; Van der Rest, M.; Prockop, D.J.
    Type I procollagen N-proteinase from chick embryo tendons. Purification of a new 500-kDa form of the enzyme and identification of the catalytically active polypeptides (1989), J. Biol. Chem., 264, 11336-11345.
    View publication on PubMed

General Stability

General Stability Organism
Ca2+ stabilizes Gallus gallus
Ca2+ stabilizes Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Bos taurus
1,10-phenanthroline 100% inhibition at 1 mM Gallus gallus
2-mercaptoethanol
-
Bos taurus
2-mercaptoethanol 78% inhibition at 1 mM Gallus gallus
concanavalin A
-
Bos taurus
concanavalin A 74% inhibition at 50 micromol/ml Gallus gallus
Cu2+
-
Bos taurus
Cu2+ 63% inhibition at 0.1 mM Gallus gallus
EDTA
-
Bos taurus
EDTA 100% inhibition at 7.5 mM Gallus gallus
Fetal bovine serum
-
Bos taurus
Fetal bovine serum 56% inhibition at concentration of 2% Gallus gallus
heparan sulfate
-
Bos taurus
heparan sulfate 50% inhibition at 0.05 micromol/ml Gallus gallus
heparin 76% inhibition at 0.05 micromol/ml Bos taurus
heparin 76% inhibition at 0.05 micromol/ml Gallus gallus
NaCl 40% inhibition at 0.3 M Gallus gallus
Ni2+
-
Bos taurus
Ni2+ 42% inhibition at 0.1 mM Gallus gallus
phosphate
-
Bos taurus
phosphate 100% inhibition at 10 mM sodium phosphate Gallus gallus
Poly-L-aspartate
-
Bos taurus
Poly-L-aspartate 81% inhibition at 10 micromol/ml Gallus gallus
Poly-L-glutamate
-
Bos taurus
Poly-L-glutamate 89% inhibition at 10 micromol/ml Gallus gallus
SDS
-
Bos taurus
SDS 84% inhibition at 50 micromol/ml Gallus gallus
Tris-HCl 80% inhibition at 0.3 M Gallus gallus
Zn2+
-
Bos taurus
Zn2+ 92% inhibition at 0.1 mM Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000054
-
procollagen I
-
Gallus gallus

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ increase of activity Gallus gallus
Ba2+ increase of activity Bos taurus
Ba2+ 70% as effective as Ca2+ Gallus gallus
Ca2+ required for maximal activity Gallus gallus
Ca2+ required for maximal activity Bos taurus
Ca2+ optimal activity at 5 to 10 mM Gallus gallus
Ca2+ optimal activity at 5 to 10 mM Bos taurus
Mg2+ increase of activity Gallus gallus
Mg2+ increase of activity Bos taurus
Mg2+ 30% as effective as Ca2+ Gallus gallus
Mn2+ increase of activity Gallus gallus
Mn2+ increase of activity Bos taurus
Mn2+ 70% as effective as Ca2+ Gallus gallus
Sr2+ increase of activity Gallus gallus
Sr2+ increase of activity Bos taurus
Sr2+ 85% as effective as Ca2+ Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
500000
-
gel filtration Gallus gallus
500000
-
gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
procollagen I + H2O Gallus gallus
-
?
-
?
procollagen I + H2O Bos taurus
-
?
-
?
procollagen II + H2O Gallus gallus
-
?
-
?
procollagen II + H2O Bos taurus
-
?
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Gallus gallus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification glycoprotein Gallus gallus
side-chain modification glycoprotein Bos taurus

Purification (Commentary)

Purification (Comment) Organism
-
Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
skin
-
Bos taurus
-
tendon
-
Gallus gallus
-
tendon
-
Bos taurus
-

Storage Stability

Storage Stability Organism
4 to -20°C, 0.05 M Tris-HCl, 0.75 M NaCl, 0.02% Brij 35, 0.01% NaN3, pH 7.5, 1-2 months, 90% activity recovered Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
procollagen I + H2O not procollagen III Gallus gallus pCCollagen I + oligopeptide partially processed procollagen containing only the C-propeptides ?
procollagen I + H2O not procollagen III Bos taurus pCCollagen I + oligopeptide partially processed procollagen containing only the C-propeptides ?
procollagen I + H2O
-
Gallus gallus ?
-
?
procollagen I + H2O
-
Bos taurus ?
-
?
procollagen II + H2O
-
Gallus gallus pCCollagen II + oligopeptide partially processed procollagen containing only the C-propeptides ?
procollagen II + H2O
-
Bos taurus pCCollagen II + oligopeptide partially processed procollagen containing only the C-propeptides ?
procollagen II + H2O
-
Gallus gallus ?
-
?
procollagen II + H2O
-
Bos taurus ?
-
?

Subunits

Subunits Comment Organism
oligomer SDS-PAGE Gallus gallus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0967
-
procollagen I
-
Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9 8.3 procollagen I Gallus gallus

pH Range

pH Minimum pH Maximum Comment Organism
5.5
-
inactive below, procollagen I as substrate Gallus gallus
5.5
-
inactive below, procollagen I as substrate Bos taurus
6.8 9.5 procollagen I as substrate, 20% of optimum activity at pH 6.8 and pH 9.5 Gallus gallus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 10 type I/II procollagen N-proteinase Gallus gallus