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Literature summary for 3.4.24.12 extracted from
- Hatching enzyme of the sea urchin Strongylocentrotus purpuratus (1976), Methods Enzymol., 45, 354-373.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
Strongylocentrotus purpuratus |  |
| Cr2+ | - |
Strongylocentrotus purpuratus | |
| Cu2+ | - |
Strongylocentrotus purpuratus | |
| EDTA | - |
Strongylocentrotus purpuratus | |
| Fe2+ | - |
Strongylocentrotus purpuratus | |
| Zn2+ | - |
Strongylocentrotus purpuratus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Strongylocentrotus purpuratus | |
| 0.84 | - |
dimethylated casein | - |
Strongylocentrotus purpuratus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | optimal concentration near 10 mM for the digestion of the fertilization envelope, 10-25 mM for casein digestion | Strongylocentrotus purpuratus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28500 | - |
1 * 28500, SDS-PAGE | Strongylocentrotus purpuratus |
| 30000 | - |
- |
Strongylocentrotus purpuratus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Strongylocentrotus purpuratus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Strongylocentrotus purpuratus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzyloxycarbonyl-Glu-Phe + H2O | - |
Strongylocentrotus purpuratus | benzyloxycarbonyl-Glu + Phe | - |
? | |
| Benzyloxycarbonyl-Glu-Tyr + H2O | - |
Strongylocentrotus purpuratus | Benzyloxycarbonyl-Glu + Tyr | - |
? | |
| casein + H2O | modified casein | Strongylocentrotus purpuratus | ? | - |
? | |
| dimethylated casein + H2O | - |
Strongylocentrotus purpuratus | ? | - |
? | |
| Dimethylcasein + H2O | - |
Strongylocentrotus purpuratus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 28500, SDS-PAGE | Strongylocentrotus purpuratus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | - |
- |
Strongylocentrotus purpuratus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
half-life: 0.32 h, 10% glycerol v/v increase half-life to 12 h | Strongylocentrotus purpuratus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.038 | - |
dimethylated casein | - |
Strongylocentrotus purpuratus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
- |
Strongylocentrotus purpuratus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
5 min, more than 95% loss of activity | Strongylocentrotus purpuratus |
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