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Literature summary for 3.4.23.B6 extracted from

  • Song, C.; Micoli, K.; Bauerova, H.; Pichova, I.; Hunter, E.
    Amino acid residues in the cytoplasmic domain of the Mason-Pfizer monkey virus glycoprotein critical for its incorporation into virions (2005), J. Virol., 79, 11559-11568.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.23.B6

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in COS-1 cells Mason-Pfizer monkey virus

Protein Variants

Protein Variants Comment Organism
H21A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Mason-Pfizer monkey virus
Q9A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme, and mutant virions show reduced incorporation of the viral glycoprotein Env during virus assembly Mason-Pfizer monkey virus
Q9A/V20A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme, and mutant virions show reduced incorporation of the viral glycoprotein Env during virus assembly Mason-Pfizer monkey virus
V20A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme, and mutant virions show reduced incorporation of the viral glycoprotein Env during virus assembly Mason-Pfizer monkey virus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.136
-
 IQVHYHRLEQ pH 5.3, 37°C, wild-type enzyme Mason-Pfizer monkey virus
0.299
-
 IQVHYHRLEQ pH 5.3, 37°C, mutant V20A Mason-Pfizer monkey virus
1.076
-
 IQVHYHRLEQ pH 5.3, 37°C, mutant H21A Mason-Pfizer monkey virus

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl activates at 0.3 M Mason-Pfizer monkey virus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mason-Pfizer monkey virus the enzyme is involved in incorporation of the viral glycoprotein Env during virus assembly, the enzyme also converts the cell-associated transmembrane protein TM to a virus-associated gp20, effect of mutation in the cytoplasmic domain of TM, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mason-Pfizer monkey virus
-
 M-PMV
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
IQVHYHRLEQ + H2O gp22-derived peptide, peptide derived from the cytoplasmic domain of transmembrane protein TM Mason-Pfizer monkey virus IQVH + YHRLEQ
-
 ?
additional information the enzyme is involved in incorporation of the viral glycoprotein Env during virus assembly, the enzyme also converts the cell-associated transmembrane protein TM to a virus-associated gp20, effect of mutation in the cytoplasmic domain of TM, overview Mason-Pfizer monkey virus ?
-
 ?

Synonyms

Synonyms Comment Organism
M-PMV PR
-
 Mason-Pfizer monkey virus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mason-Pfizer monkey virus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.053
-
 IQVHYHRLEQ pH 5.3, 37°C, mutant V20A Mason-Pfizer monkey virus
0.175
-
 IQVHYHRLEQ pH 5.3, 37°C, mutant H21A Mason-Pfizer monkey virus
0.43
-
 IQVHYHRLEQ pH 5.3, 37°C, wild-type enzyme Mason-Pfizer monkey virus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3
-
 assay at Mason-Pfizer monkey virus