Cloned (Comment) | Organism |
---|---|
cloned directly from proviral DNA of the infectious viral stock SIVmac251-32H(11/88 pool). SIVmac251-32H proteinase and ist flanking pol sequences are expressed in Escherichia coli as a fusion protein with most of the T7 bacteriophage gene 10 protein. The expressed protein forms cytoplasmic inclusion bodies which are solubilized in 8 M urea, and the recombinant enzyme is refolded, yielding active self-processing enzyme | Simian immunodeficiency virus |
Crystallization (Comment) | Organism |
---|---|
- |
Simian immunodeficiency virus |
General Stability | Organism |
---|---|
microcrystals are stable under mild acidic conditions | Simian immunodeficiency virus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
pepstatin A | 0.01-0.1 mM | Simian immunodeficiency virus | |
ZnCl2 | 1 mM, no detectable activity | Simian immunodeficiency virus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | 1 M NaCl, rate of substrate cleavage increases up to 4fold | Simian immunodeficiency virus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
10000 | - |
x * 10000, SDS-PAGE | Simian immunodeficiency virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Simian immunodeficiency virus | - |
mac251-32H | - |
Simian immunodeficiency virus mac251-32H | - |
mac251-32H | - |
Purification (Comment) | Organism |
---|---|
- |
Simian immunodeficiency virus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
19.8 | - |
- |
Simian immunodeficiency virus |
Storage Stability | Organism |
---|---|
-80°C, in 10% glycerol, no loss of activity after 12 months | Simian immunodeficiency virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Lys-Ala-Arg-Val-Nle-(NO2)Phe-Glu-Ala-Nle-Gly + H2O | - |
Simian immunodeficiency virus | Lys-Ala-Arg-Val-Nle + (NO2)Phe-Glu-Ala-Nle-Gly | - |
? | |
Lys-Ala-Arg-Val-Nle-(NO2)Phe-Glu-Ala-Nle-Gly + H2O | - |
Simian immunodeficiency virus mac251-32H | Lys-Ala-Arg-Val-Nle + (NO2)Phe-Glu-Ala-Nle-Gly | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 10000, SDS-PAGE | Simian immunodeficiency virus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.3 | - |
- |
Simian immunodeficiency virus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Simian immunodeficiency virus | - |
- |
7.4 |