Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NaCl | assay at 3 M, no activity at salt concentrations of 0.2-0.4 M Na. In vitro dimerization of the protease domain is inducible at high salt concentrations. This effect might be caused by a hydrophobic dimerization interface, which under high ionic strength disfavors the monomeric state | Simian foamy virus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Simian foamy virus |
expression in Escherichia coli | prototype foamy virus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
84500 | - |
gel filtration | prototype foamy virus |
86500 | - |
1 * 86500, calculated | prototype foamy virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
prototype foamy virus | - |
- |
- |
Simian foamy virus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GB1-GFP + H2O | peptide substrate, harboring the simian foamy virus mac reverse transcriptase-viral integrase cleavage site of the Pol polyprotein, ATQGSYVVHCNTTP, between immunoglobulin binding domain B1 of streptococcal protein G, GB1, and green fluorescent protein, GFP | Simian foamy virus | ? | cleavage occurs at YVVH-CNTT | ? | |
GB1-GFP + H2O | peptide substrate, harboring the simian foamy virus mac reverse transcriptase-viral integrase cleavage site of the Pol polyprotein, ATQGSYVVHCNTTP, between immunoglobulin binding domain B1 of streptococcal protein G, GB1, and green fluorescent protein, GFP | prototype foamy virus | ? | cleavage occurs at YVVH-CNTT | ? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 86500, calculated | prototype foamy virus |
More | under native conditions, no detection of dimer | prototype foamy virus |