Literature summary for 3.4.23.51 extracted from

Kumarevel, T.; Tanaka, T.; Bessho, Y.; Shinkai, A.; Yokoyama, S.
Crystal structure of hydrogenase maturating endopeptidase HycI from Escherichia coli (2009), Biochem. Biophys. Res. Commun., 389, 310-314.

Search for more literature for 3.4.23.51

Cloned(Commentary)

Cloned (Comment)	Organism
-	Escherichia coli K-12

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using 28% (w/v) polyethylene glycol 400, 0.2 M CaCl2, and 0.1 M Na-HEPES (pH 7.5), at 20°C	Escherichia coli K-12

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
17000	-	-	Escherichia coli K-12

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli K-12	P0AEV9	-	-

Purification (Commentary)

Purification (Comment)	Organism
HisTrap HP5 column chromatography, HiPrep column chromatography, and Superdex 200 gel filtration	Escherichia coli K-12

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
precursor of the large subunit of hydrogenase 3 + H2O	the HycI endopeptidase is involved in the C-terminal processing of the large subunit of hydrogenase 3 (HycE)	Escherichia coli K-12	?	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 17000, HycI exists as a monomer in solution, X-ray crystallography	Escherichia coli K-12

Synonyms

Synonyms	Comment	Organism
HycI endopeptidase	-	Escherichia coli K-12

pI Value

Organism	Comment	pI Value Maximum	pI Value
Escherichia coli K-12	calculated from amino acid sequence	-	3.7

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Release 2023.1 (January 2023)