Literature summary for 3.4.23.5 extracted from

Rodriguez-Siordia, I.; Rojo-Arreola, L.; Navarrete Del Toro, M.L.A.; Garcia-Carreno, F.
American lobster Cathepsin D, an aspartic peptidase resistant to proteolysis and active in organic solvents, non-ionic detergents and salts (2018), Int. J. Biol. Macromol., 107, 1501-1509 .

Search for more literature for 3.4.23.5

Activating Compound

Activating Compound	Comment	Organism	Structure
ethanol	in 10% (v/v) ethanol, the enzyme significantly increases its activity by 50%	Homarus americanus
Isopropanol	the presence of 15% (v/v) isopropanol generates a significant increase of 80% on enzyme activity	Homarus americanus
methanol	exposure of the enzyme to 5-15% (v/v) methanol produces 50% increase in enzyme activity	Homarus americanus

General Stability

General Stability	Organism
the non-ionic detergents Tween-20 and Triton X-100 stabilize the enzyme activity. The enzyme is active until 2.5 M urea and is resistant to proteolysis by papain and renin. Glycerol (2.5-30% (v/v)) shows no stabilization effect on the activity of the enzyme. In 25% (v/v) methanol, the enzyme retains 100% of the activity	Homarus americanus

Inhibitors

Inhibitors	Comment	Organism	Structure
DMSO	in the presence of 10% (v/v) DMSO a significant decrease in activity to 40% is observed. In 25% (v/v) DMSO the enzyme maintains 20% of the activity. Activity is completely lost when the enzyme is exposed to DMSO at concentration above 35% (v/v)	Homarus americanus
ethanol	at 25 and 35% (v/v) ethanol, enzyme activity decreases to 60 and 40%, respectively. In 50% ethanol, the enzyme completely loses its activity	Homarus americanus
Isopropanol	at 25% (v/v) isopropanol, the enzyme activity drops to 50%, enzyme activity is completely lost at 35% (v/v)	Homarus americanus
methanol	the enzyme loses activity in the presence of more than 35% (v/v) methanol	Homarus americanus
NaCl	purified enzyme retains more than 50% of proteolytic activity in final concentration of 1 M NaCl and 20% in 1.5 M NaCl	Homarus americanus
Triton X-100	60% residual activity at 0.001-0.01% (v/v) Triton X-100, below 20% residual activity at 0.0005% or 0.05-0.1% (v/v) Triton X-100	Homarus americanus

Organism

Organism	UniProt	Comment	Textmining
Homarus americanus	-	American lobster	-

Purification (Commentary)

Purification (Comment)	Organism
pepstatin A-agarose column chromatography, NAP-5 Sephadex G25 gel filtration, and UNO Q1 column chromatography	Homarus americanus

Source Tissue

Source Tissue	Comment	Organism	Textmining
gastric juice	-	Homarus americanus	-

Storage Stability

Storage Stability	Organism
25°C, purified and concentrated enzyme (0.005 mg/ml) at pH 4.5, 7 days, no loss of activity	Homarus americanus
25°C, purified and low-concentrated enzyme (0.00005 mg/ml) at pH 4.5, 8 h, complete loss of activity	Homarus americanus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
((7-methoxycoumarin-4-yl)acetyl)-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Lys-(2,4-dinitrophenyl)-D-Arg-amide + H2O	-	Homarus americanus	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 28000, SDS-PAGE	Homarus americanus

Synonyms

Synonyms	Comment	Organism
cathepsin D1	-	Homarus americanus
CD1	-	Homarus americanus

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Release 2023.1 (January 2023)