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Literature summary for 3.4.23.5 extracted from
- Purification and partial characterization of ostrich skeletal muscle cathepsin D and its activity during meat maturation (2011), Meat Sci., 87, 196-201.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,2-epoxy-3-(4-nitrophenoxy)propane | - |
Struthio camelus |  |
| 4-bromophenacyl bromide | - |
Struthio camelus | |
| dithiothreitol | strong inhibitor | Struthio camelus | |
| additional information | not inhibited by diazoacetyl-DL-norleucine methyl ester | Struthio camelus | |
| pepstatin A | strong inhibitor | Struthio camelus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03154 | - |
hemoglobin | in 0.05 M sodium acetate buffer, pH 3.5, at 45°C | Struthio camelus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 14000 | - |
1 * 29100 + 1 * 14000 | Struthio camelus |
| 29100 | - |
1 * 29100 + 1 * 14000 | Struthio camelus |
| 45000 | - |
SDS-PAGE | Struthio camelus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Struthio camelus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| pepstatin A agarose column chromatography | Struthio camelus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| skeletal muscle | - |
Struthio camelus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the specific activities of the crude extract and after 273.4fold purification are 41.5 and 11345.6 units/mg, respectively | Struthio camelus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, storage medium, 30 days, cathepsin D remains substantially active with an average remaining activity of 112% at day 30 (mean value from 5 ostriches) | Struthio camelus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Hemoglobin + H2O | - |
Struthio camelus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterodimer | 1 * 29100 + 1 * 14000 | Struthio camelus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Struthio camelus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | - |
Struthio camelus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Struthio camelus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 5 | cathepsin D shows low activity at pH 3 and 45% remaining activity is observed up to pH 5.0 | Struthio camelus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00000307 | - |
pepstatin A | in 0.05 M sodium acetate buffer, pH 3.5, at 45°C | Struthio camelus | |
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