Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.23.49 extracted from

  • da Silva, R.R.; de Oliveira, L.C.; Juliano, M.A.; Juliano, L.; de Oliveira, A.H.; Rosa, J.C.; Cabral, H.
    Biochemical and milk-clotting properties and mapping of catalytic subsites of an extracellular aspartic peptidase from basidiomycete fungus Phanerochaete chrysosporium (2017), Food Chem., 225, 45-54 .
    View publication on PubMed
Search for more literature for 3.4.23.49

Application

Application Comment Organism
food industry the peptidase shows maximal milk clotting activity at 60-65 °C and maintenance of enzymatic activity above 80% in the presence of 20 mM CaCl2 Phanerodontia chrysosporium

Inhibitors

Inhibitors Comment Organism Structure
Al3+ 1 mM, 47% residual activity Phanerodontia chrysosporium
Cu2+ 1 mM, 77% residual activity Phanerodontia chrysosporium
guanidine hydrochloride 50 mM, 40% residual activity Phanerodontia chrysosporium
pepstatin A 5 mM, complete loss of activity Phanerodontia chrysosporium
SDS 0.02%, complete loss of activity Phanerodontia chrysosporium
Tween 20 up to 1%, 80% residual activity Phanerodontia chrysosporium
Urea 50 mM, 72% residual activity Phanerodontia chrysosporium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00012
-
 2-aminobenzoyl-KQRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 4.5, 40°C Phanerodontia chrysosporium
0.00024
-
 2-aminobenzoyl-KLRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 4.5, 40°C Phanerodontia chrysosporium
0.0003
-
 2-aminobenzoyl-KMRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 4.5, 40°C Phanerodontia chrysosporium
0.00043
-
 2-aminobenzoyl-KNRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 4.5, 40°C Phanerodontia chrysosporium
0.00064
-
 2-aminobenzoyl-KLKSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 4.5, 40°C Phanerodontia chrysosporium

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Phanerodontia chrysosporium
-
-

Organism

Organism UniProt Comment Textmining
Phanerodontia chrysosporium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Phanerodontia chrysosporium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
750
-
 pH 4.5, 40°C Phanerodontia chrysosporium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminobenzoyl-KLKSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine + H2O
-
 Phanerodontia chrysosporium 2-aminobenzoyl-KLK + SSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine
-
 ?
2-aminobenzoyl-KLRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine + H2O
-
 Phanerodontia chrysosporium 2-aminobenzoyl-KLR + SSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine
-
 ?
2-aminobenzoyl-KLRSSVQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine + H2O
-
 Phanerodontia chrysosporium 2-aminobenzoyl-KLR + SSVQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine
-
 ?
2-aminobenzoyl-KMRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine + H2O
-
 Phanerodontia chrysosporium 2-aminobenzoyl-KMR + SSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine
-
 ?
2-aminobenzoyl-KNRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine + H2O
-
 Phanerodontia chrysosporium 2-aminobenzoyl-KNR + SSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine
-
 ?
2-aminobenzoyl-KQRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine + H2O
-
 Phanerodontia chrysosporium 2-aminobenzoyl-KQR + SSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine
-
 ?
additional information enzyme displays restriction of catalysis at the S1 subsite, with a preference for lysine, arginine, leucine, tyrosine, and phenylalanine residues. No hydrolysis of the substrate peptide is observed with amino acids A, D, E, H, I, M, N, P, Q, S, T, V, or W in the S1 position. At S2 and S1' subsites, the enzyme exhibits a good acceptance of amino acids L, M, E, D, R and S, Y, Q, respectively Phanerodontia chrysosporium ?
-
 ?

Subunits

Subunits Comment Organism
? x * 38000, SDS-PAGE Phanerodontia chrysosporium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Phanerodontia chrysosporium

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
65
-
 50% of maximum activity Phanerodontia chrysosporium
70
-
 40% of maximum activity Phanerodontia chrysosporium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 stable for 1 h Phanerodontia chrysosporium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.64
-
 2-aminobenzoyl-KMRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 4.5, 40°C Phanerodontia chrysosporium
0.8
-
 2-aminobenzoyl-KNRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 4.5, 40°C Phanerodontia chrysosporium
0.824
-
 2-aminobenzoyl-KQRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 4.5, 40°C Phanerodontia chrysosporium
1.15
-
 2-aminobenzoyl-KLRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 4.5, 40°C Phanerodontia chrysosporium
1.54
-
 2-aminobenzoyl-KLKSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 4.5, 40°C Phanerodontia chrysosporium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Phanerodontia chrysosporium

pH Range

pH Minimum pH Maximum Comment Organism
5.5
-
 46% of maximum activity Phanerodontia chrysosporium

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 8
-
 Phanerodontia chrysosporium

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1860
-
 2-aminobenzoyl-KNRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 4.5, 40°C Phanerodontia chrysosporium
2000
-
 2-aminobenzoyl-KQRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 4.5, 40°C Phanerodontia chrysosporium
2133
-
 2-aminobenzoyl-KMRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 4.5, 40°C Phanerodontia chrysosporium
2406
-
 2-aminobenzoyl-KLKSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 4.5, 40°C Phanerodontia chrysosporium
4732
-
 2-aminobenzoyl-KLRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 4.5, 40°C Phanerodontia chrysosporium
4792
-
 2-aminobenzoyl-KLRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 4.5, 40°C Phanerodontia chrysosporium