Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.23.49 extracted from

  • Salunkhe, S.S.; Raiker, V.A.; Rewanwar, S.; Kotwal, P.; Kumar, A.; Padmanabhan, S.
    Enhanced fluorescent properties of an OmpT site deleted mutant of green fluorescent protein (2010), Microb. Cell Fact., 9, 26.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
biotechnology OmpT protease could be a possible factor responsible for reducing the expression of GFP at 37°C for wildtype GFP clone in K12 hosts like DH5alpha, JM109, LE 392 Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Zn
-
Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
OmpT proteolytic site of the GFP + H2O the construction of two GFP variants with modified putative OmpT proteolytic sites by site directed mutagenesis is described. Such modified genes upon arabinose induction exhibit varied degrees of GFP fluorescence. While the mutation of K79G/R80A close to the fluorophore results in dramatic loss of fluorescence activity, the modification of K214A/R215A results in four fold enhanced fluorescence of GFP K214A/R215 Escherichia coli ?
-
?

Synonyms

Synonyms Comment Organism
ompT
-
Escherichia coli

Expression

Organism Comment Expression
Escherichia coli OmpT expression levels are higher at 37°C than at 30°C up