Application | Comment | Organism |
---|---|---|
biotechnology | OmpT protease could be a possible factor responsible for reducing the expression of GFP at 37°C for wildtype GFP clone in K12 hosts like DH5alpha, JM109, LE 392 | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Zn | - |
Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
OmpT proteolytic site of the GFP + H2O | the construction of two GFP variants with modified putative OmpT proteolytic sites by site directed mutagenesis is described. Such modified genes upon arabinose induction exhibit varied degrees of GFP fluorescence. While the mutation of K79G/R80A close to the fluorophore results in dramatic loss of fluorescence activity, the modification of K214A/R215A results in four fold enhanced fluorescence of GFP K214A/R215 | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ompT | - |
Escherichia coli |
Organism | Comment | Expression |
---|---|---|
Escherichia coli | OmpT expression levels are higher at 37°C than at 30°C | up |