Application | Comment | Organism |
---|---|---|
biotechnology | utilization of outer-membrane endoprotease OmpT variants as processing enzymes for production of peptides from designer fusion proteins, e.g. useful in motilin production, overview | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
overexpression of wild-type and mutant enzymes strain W3110 M25 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D97A | site-directed mutagenesis, mutant shows altered cleavage specificity compared to the wild-type enzyme, substrate specificity with fusion protein, overview | Escherichia coli |
D97C | site-directed mutagenesis, mutant shows altered cleavage specificity compared to the wild-type enzyme, substrate specificity with fusion protein, overview | Escherichia coli |
D97F | site-directed mutagenesis, mutant shows altered cleavage specificity compared to the wild-type enzyme, substrate specificity with fusion protein, overview | Escherichia coli |
D97H | site-directed mutagenesis, mutant shows altered cleavage specificity compared to the wild-type enzyme, preference for human calcitonin precursor, substrate specificity with fusion protein, overview | Escherichia coli |
D97L | site-directed mutagenesis, mutant shows altered cleavage specificity compared to the wild-type enzyme, preference for human adrenocarticotropic hormone, substrate specificity with fusion protein, overview | Escherichia coli |
D97M | site-directed mutagenesis, mutant shows altered cleavage specificity compared to the wild-type enzyme, preference for a fusion peptide substrate with the sequence R-R-R-A-R*-motilin, substrate specificity with fusion protein, overview | Escherichia coli |
D97N | site-directed mutagenesis, mutant shows altered cleavage specificity compared to the wild-type enzyme, substrate specificity with fusion protein, overview | Escherichia coli |
D97Q | site-directed mutagenesis, mutant shows altered cleavage specificity compared to the wild-type enzyme, substrate specificity with fusion protein, overview | Escherichia coli |
D97S | site-directed mutagenesis, mutant shows altered cleavage specificity compared to the wild-type enzyme, substrate specificity with fusion protein, overview | Escherichia coli |
D97T | site-directed mutagenesis, mutant shows altered cleavage specificity compared to the wild-type enzyme, substrate specificity with fusion protein, overview | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
outer membrane | - |
Escherichia coli | 19867 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
Has a virtual requirement for Arg in the P1 position and a slightly less stringent preference for this residue in the P1' position, which can also contain Lys, Gly or Val. | the consensus sequence of OmpT is R/K-A, the enzyme is highly selective for a basic amino acid residue at position P1, but less exclusive at P1', where several non-basic amino acids are tolerated, enzyme residue Asp97 is responsible for interaction with P1' position substrate residue | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a fusion-motilin peptide + H2O | proteolytic cleavage by mutant D97M at R-R-R-A-R*-motilin | Escherichia coli | ? | - |
? | |
dynorphin A + H2O | proteolytic cleavage, commercial peptide substrate | Escherichia coli | ? | - |
? | |
human adrenocorticotropic hormone + H2O | proteolytic cleavage by mutant D97L at Ser24, release of the hormone | Escherichia coli | ? | - |
? | |
human calcitonin precursor + H2O | proteolytic cleavage by mutant D97H at an N-terminal Cys | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
endoprotease | - |
Escherichia coli |
ompT | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |