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Literature summary for 3.4.23.49 extracted from
- An analysis of target preferences of Escherichia coli outer-membrane endoprotease OmpT for use in therapeutic peptide production: efficient cleavage of substrates with basic amino acids at the P4 and P6 positions (2002), Biotechnol. Appl. Biochem., 36, 77-84.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | use in therapeutic peptide production, efficient cleavage of substrates with basic amino acids at the P4 and P6 positions, able to cleave efficiently a fusion protein carrying human glucagon-like peptide I, releases mature protein from an Escherichia coli expressed fusion protein carrying human atrial natriuretic peptide, a drug for acute congestive heart failure | Escherichia coli |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
ELRLYKRHHG | pH 7.0, 25°C | Escherichia coli |  |
| 0.56 | - |
ELELYKRHHG | pH 7.0, 25°C | Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | integral membrane protease, outer membrane, membrane bound | Escherichia coli | 16020 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Escherichia coli | cleaves peptides between two consecutive basic amino acids | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| no activity in Escherichia coli | - |
M25 | - |
| no activity in Escherichia coli W3110 / ATCC 27325 | - |
M25 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dynorphin A + H2O | - |
Escherichia coli | ? | - |
? | |
| ELELYKRHHG + H2O | - |
Escherichia coli | ELELYK + RHHG | - |
? | |
| ELRLYKRHHG + H2O | - |
Escherichia coli | ELRLYK + RHHG | - |
? | |
| additional information | cleaves peptides between two consecutive basic amino acids | Escherichia coli | ? | - |
? | |
| RLELYKRHHG + H2O | - |
Escherichia coli | RLELYK + RHHG | - |
? | |
| RLRLYKRHHG + H2O | - |
Escherichia coli | RLRLYK + RHHG | - |
? | |
| RSANP + H2O | atrial natriuretic peptide | Escherichia coli | ANP + ? | - |
? | |
| RSANPR + H2O | atrial natriuretic peptide | Escherichia coli | ANP + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ompT | - |
Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 372 | - |
RLRLYKRHHG | pH 7.0, 25°C | Escherichia coli | |
| 378 | - |
RLELYKRHHG | pH 7.0, 25°C | Escherichia coli | |
| 660 | - |
ELELYKRHHG | pH 7.0, 25°C | Escherichia coli | |
| 3720 | - |
ELRLYKRHHG | pH 7.0, 25°C | Escherichia coli | |
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Release 2023.1 (January 2023)
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