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Literature summary for 3.4.23.43 extracted from

  • de Bentzmann, S.; Aurouze, M.; Ball, G.; Filloux, A.
    FppA, a novel Pseudomonas aeruginosa prepilin peptidase involved in assembly of type IVb pili (2006), J. Bacteriol., 188, 4851-4860.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.23.43

Cloned(Commentary)

Cloned (Comment) Organism
gene fppA, DNA and amino acid sequence determination and analysis, expression of wild-type and mutants in strain PAO1, method optimization Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
D17A site-directed mutagenesis, inactive mutant Pseudomonas aeruginosa
D17A/D78A site-directed mutagenesis, inactive mutant Pseudomonas aeruginosa
D78A site-directed mutagenesis, inactive mutant Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
type IV Flp1 prepilin + H2O Pseudomonas aeruginosa maturation of pilin type IV, the enzyme is involved in the assembly of type IVb pili required for motility and attachment, overview type IV Flp1 pilin + type IV Flp1 prepilin leader peptide
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 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
 gene fppA
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Reaction

Reaction Comment Organism Reaction ID
typically cleaves a -Gly-/-Phe- bond to release an N-terminal, basic peptide of 5-8 residues from type IV prepilin, and then N-methylates the new N-terminal amino group, the methyl donor being S-adenosyl-L-methionine Asp17 and Asp78 are the catalytic residues Pseudomonas aeruginosa
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
type IV Flp1 prepilin + H2O
-
 Pseudomonas aeruginosa type IV Flp1 pilin + type IV Flp1 prepilin leader peptide
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 ?
type IV Flp1 prepilin + H2O maturation of pilin type IV, the enzyme is involved in the assembly of type IVb pili required for motility and attachment, overview Pseudomonas aeruginosa type IV Flp1 pilin + type IV Flp1 prepilin leader peptide
-
 ?