Literature summary for 3.4.23.43 extracted from

Szabo, Z.; Albers, S.V.; Driessen, A.J.
Active-site residues in the type IV prepilin peptidase homologue PibD from the archaeon Sulfolobus solfataricus (2006), J. Bacteriol., 188, 1437-1443.

Search for more literature for 3.4.23.43

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli in inner membrane vesicles	Saccharolobus solfataricus

Protein Variants

Protein Variants	Comment	Organism
D157A	site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme	Saccharolobus solfataricus
D173A	site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme	Saccharolobus solfataricus
D180A	site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme	Saccharolobus solfataricus
D187A	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	Saccharolobus solfataricus
D187A/D188A	site-directed mutagenesis, inactive mutant	Saccharolobus solfataricus
D188A	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	Saccharolobus solfataricus
D207N	site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme	Saccharolobus solfataricus
D23A	site-directed mutagenesis, inactive mutant	Saccharolobus solfataricus
D30A	site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme	Saccharolobus solfataricus
D80A	site-directed mutagenesis, inactive mutant	Saccharolobus solfataricus
D80E	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	Saccharolobus solfataricus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	membrane topology, overview	Saccharolobus solfataricus	16020	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
preflagellin + H2O	Saccharolobus solfataricus	-	flagellin + preflagellin leader peptide	-	?
prepilin type IV + H2O	Saccharolobus solfataricus	-	pilin type IV + prepilin type IV leader peptide	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	Q6IFS8	gene pibD	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme from Escherichia coli inner membrane vesicles	Saccharolobus solfataricus

Reaction

Reaction	Comment	Organism	Reaction ID
typically cleaves a -Gly-/-Phe- bond to release an N-terminal, basic peptide of 5-8 residues from type IV prepilin, and then N-methylates the new N-terminal amino group, the methyl donor being S-adenosyl-L-methionine	Asp80 and Asp23 are the catalytic residues	Saccharolobus solfataricus	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the enzyme cleaves sugar-binding proteins unique to Sulfolobus solfataricus	Saccharolobus solfataricus	?	-	?
preflagellin + H2O	-	Saccharolobus solfataricus	flagellin + preflagellin leader peptide	-	?
prepilin type IV + H2O	-	Saccharolobus solfataricus	pilin type IV + prepilin type IV leader peptide	-	?

Synonyms

Synonyms	Comment	Organism
More	the enzyme belongs to the family of aspartic proteases	Saccharolobus solfataricus
PibD	-	Saccharolobus solfataricus
preflagellin peptidase	-	Saccharolobus solfataricus
type IV prepilin peptidase	-	Saccharolobus solfataricus

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Release 2023.1 (January 2023)