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Literature summary for 3.4.23.40 extracted from

  • Verissimo, P.; Faro, C.; Moir, A.J.; Lin, Y.; Tang, J.; Pires, E.
    Purification, characterization and partial amino acid sequencing of two new aspartic proteinases from fresh flowers of Cynara cardunculus L. (1996), Eur. J. Biochem., 235, 762-768.
    View publication on PubMed
Search for more literature for 3.4.23.40

Inhibitors

Inhibitors Comment Organism Structure
diazoacetyl-norleucine methyl ester
-
 Cynara cardunculus
pepstatin
-
 Cynara cardunculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.081
-
 Leu-Ser-Phe(NO2)-Ahx-Ala-Leu-OMe pH 4.7, 37°C Cynara cardunculus
0.108
-
 Lys-Pro-Ala-Glu-Phe-Phe-Phe(NO2)-Ala-Leu pH 4.7, 37°C Cynara cardunculus
0.11
-
 Lys-Pro-Ala-Glu-Phe-Phe-Phe(NO2)-Ala-Leu pH 4.7, 37°C Cynara cardunculus
0.64
-
 Leu-Ser-Phe(NO2)-Ahx-Ala-Leu-OMe pH 4.7, 37°C Cynara cardunculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15000
-
 x * 31000, and x * 15000, SDS-PAGE Cynara cardunculus
15000
-
 x * 34000, and x * 15000, SDS-PAGE Cynara cardunculus
31000
-
 x * 31000, and x * 15000, SDS-PAGE Cynara cardunculus
34000
-
 x * 34000, and x * 15000, SDS-PAGE Cynara cardunculus

Organism

Organism UniProt Comment Textmining
Cynara cardunculus Q9XFX3 isoform CardA
-
Cynara cardunculus Q9XFX4 isoform CardB
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
91.8
-
 pH 4.7, 37°C Cynara cardunculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Leu-Ser-Phe(NO2)-Ahx-Ala-Leu-OMe + H2O
-
 Cynara cardunculus ?
-
 ?
Leu-Ser-Phe(NO2)-Ahx-Ala-Leu-OMe + H2O substrate of cardosin B Cynara cardunculus ?
-
 ?
Lys-Pro-Ala-Glu-Phe-Phe-Phe(NO2)-Ala-Leu + H2O
-
 Cynara cardunculus ?
-
 ?
Lys-Pro-Ala-Glu-Phe-Phe-Phe(NO2)-Ala-Leu + H2O substrate of cardosin A Cynara cardunculus ?
-
 ?
Lys-Pro-Ala-Glu-Phe-Phe-Phe(NO2)-Ala-Leu + H2O
-
 Cynara cardunculus Lys-Pro-Ala-Glu-Phe-Phe + Phe(NO2)-Ala-Leu + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 31000, and x * 15000, SDS-PAGE Cynara cardunculus
? x * 34000, and x * 15000, SDS-PAGE Cynara cardunculus

Synonyms

Synonyms Comment Organism
CardA
-
 Cynara cardunculus
cardB
-
 Cynara cardunculus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 stable up to Cynara cardunculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
13.7
-
 Leu-Ser-Phe(NO2)-Ahx-Ala-Leu-OMe pH 4.7, 37°C Cynara cardunculus
55.63
-
 Lys-Pro-Ala-Glu-Phe-Phe-Phe(NO2)-Ala-Leu pH 4.7, 37°C Cynara cardunculus
86.2
-
 Leu-Ser-Phe(NO2)-Ahx-Ala-Leu-OMe pH 4.7, 37°C Cynara cardunculus
89.4
-
 Lys-Pro-Ala-Glu-Phe-Phe-Phe(NO2)-Ala-Leu pH 4.7, 37°C Cynara cardunculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Cynara cardunculus

pH Range

pH Minimum pH Maximum Comment Organism
2 7
-
 Cynara cardunculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000001
-
 pepstatin pH 4.7, 37°C Cynara cardunculus
0.000003
-
 pepstatin pH 4.7, 37°C Cynara cardunculus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
21.3
-
 Leu-Ser-Phe(NO2)-Ahx-Ala-Leu-OMe pH 4.7, 37°C Cynara cardunculus
515.1
-
 Lys-Pro-Ala-Glu-Phe-Phe-Phe(NO2)-Ala-Leu pH 4.7, 37°C Cynara cardunculus
808.3
-
 Lys-Pro-Ala-Glu-Phe-Phe-Phe(NO2)-Ala-Leu pH 4.7, 37°C Cynara cardunculus
1066
-
 Leu-Ser-Phe(NO2)-Ahx-Ala-Leu-OMe pH 4.7, 37°C Cynara cardunculus